UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, ETIL ASETAT, DAN AIR DARI EKSTRAK ETANOL DAUN SINGKONG (Manihot esculenta Crantz) TERHADAP BAKTERI Shigella dysentriae ATCC 9361

PUTRYANI, Aprillya (2018) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, ETIL ASETAT, DAN AIR DARI EKSTRAK ETANOL DAUN SINGKONG (Manihot esculenta Crantz) TERHADAP BAKTERI Shigella dysentriae ATCC 9361. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Cassava leaves (Manihot esculenta Crantz) are plants that have been widely used in the field of medicine. Cassava leaves contain flavonoid, saponin and tannin compounds. This study aims to determine the antibacterial activity of n-hexane, ethyl acetate, and water fractions from ethanol extract of cassava leaves (Manihot esculenta Crantz) on the Shigella dysentriae ATCC 9361 bacteria and to determine the value of Minimun Inhibitory Concentration and Minimum Kill Concentration of the most active fraction of cassava leaves (Manihot esculenta Crantz) against Shigella dysentriae ATCC 9361. The method used in this research is maceration method using 70% ethanol solvent. The fractionation of the extracts was then fractionated with n-hexane, ethyl acetate, and water. Testing of Shigella dysentriae ATCC 9361 bacteria using diffusion and dilution methods. Diffusion method to find out the most active fraction then proceed with the dilution method to find out the minimum active fraction concentration. The concentrations used in the diffusion method were 50%, 25%, 12.5% and the dilution method used 50% concentration; 25%; 12.5%; 6.25%; 3.12%; 1.56%; 0.78%; 0.39%. The results showed that the n-hexane, ethyl acetate and water fractions had antibacterial activity against the Shigella dysentriae ATCC 9361. The largest inhibitory zone diameter was ethyl acetate fraction at 50% concentration, ethyl acetate fraction was the most active fraction against Shigella dysentriae ATCC 9361. so that it continues with the dilution method. The results of the dilution indicate the value of the Minimum Kill Concentration of 12.5%. Keywords: Cassava leaves (Manihot esculenta Crantz), Shigella dysentriae ATCC 9361, nhexane fraction, ethyl acetate fraction, water fraction, antibacterial

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Cassava leaves (Manihot esculenta Crantz), Shigella dysentriae ATCC 9361, nhexane fraction, ethyl acetate fraction, water fraction, antibacterial
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	22 Feb 2019 07:41
Last Modified:	22 Feb 2019 07:41
URI:	http://repo.setiabudi.ac.id/id/eprint/108

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