POTENSI ANTIBAKTERI EKSTRAK DAN FRAKSI n-HEKSANA, ETIL ASETAT, DAN AIR DARI DAUN WANGON (Olax scandens Roxb) TERHADAP Pseudomonas aeruginosa ATCC 27853

WULANDARI, Riana Desi (2018) POTENSI ANTIBAKTERI EKSTRAK DAN FRAKSI n-HEKSANA, ETIL ASETAT, DAN AIR DARI DAUN WANGON (Olax scandens Roxb) TERHADAP Pseudomonas aeruginosa ATCC 27853. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Wangon leaf (Olax scandens Roxb) is a tribal plants of olacaceae. Chemical content of wangon leaf were flavonoids, saponins, tannins and steroids that were antibacterial. The purpose of this study is to know antibacterial potential of extract, fraction of n-hexane, ethyl acetate and water from extract ethanol wangon leaf (Olax scandens Roxb) against Pseudomonas aeruginosa ATCC 27853. Wangon leaf powder was extract with soxhletation method using ethanol 96% solvent. Extract obtained was fractionated with n-hexane, ethyl acetate and water solvent. Extract and fractionation results were tested for antibacterial activity against Pseudomonas aeruginosa ATCC 27853 using diffusion method with concentration 50%, 25% and 12.5% to know the most active fraction. The most active fraction then tested for antibacterial activity with dilution method to determine the minimum inhibitory concentrations and Minimum Bactericidal Concentration with concentrations of 50%, 25%, 12.5%, 6.25%, 3.125%, 1.562%, 0.781%, 0.391%, 0.195 %, 0.098%. The ethanol extract, fraction n-hexane, ethyl acetate and water of wangon leaf (Olax scandens Roxb) had antibacterial activity against Pseudomonas aeruginosa ATCC 27853. Ethyl acetate fraction with concentration 50% was the most active fraction with a mean inhibitory diameter by 13,23±0,25 mm and a Minimum Bactericidal Concentration by 25%. Keywords : Leaf wangon (Olax scandens Roxb), antibacterial, Pseudomonas aeruginosa, diffusion and dilution

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Leaf wangon (Olax scandens Roxb), antibacterial, Pseudomonas aeruginosa, diffusion and dilution
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	25 Feb 2019 03:25
Last Modified:	25 Feb 2019 03:25
URI:	http://repo.setiabudi.ac.id/id/eprint/1136

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