Hikmah, Siti Nur (2017) AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, ETIL ASETAT DAN AIR DARI EKSTRAK ETANOL DAUN YODIUM (Jatropha multifida L.) TERHADAP Pseudomonas aeruginosa ATCC 27853. Skripsi thesis, Universitas Setia Budi Surakarta.
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Abstract
Yodium leaf (J. multifida) is a plant of Euphorbiaceae. Yodium leaf chemical constituents are alkaloids, flavonoids, saponins and tannins that are antibacterial. In this study, yodium leaf explored its use as an antibacterial against P. aeruginosa ATCC 27853. The aim of the study was to find out the effectiveness of ethanol extract, n-hexana, ethyl acetate and water fraction of yodium leaf against P. aeruginosa ATCC 27853. The extraction of yodium leaf by maceration method used ethanol 70% solvent, followed by fractionation with n-hexana, ethyl acetate and water solvents. The antibacterial activity test performed by the diffusion method and dilusion method. Diffution method by consentration ethanol extracts and fraction are used 50%, 25% and 12,5%. Dilution method by consentration ethyl acetate fraction are used 50%, 25%, 12,5%, 6,25%, 3,125%, 1,5625%, 0,78125%, dan 0,390625%. The chemical contens of the most active fraction were tested by TLC. The results showed that the ethanol extract of yodium leaf (J. multifida), of n-hexana, ethyl acetate and water fraction had antibacterial activity against P. aeruginosa ATCC 27853. Ethyl acetate fraction was the most active fraction with an average of inhibition diameter at concentrations of 50% was 14,7 mm, concentration of 25% was 14,4 mm and concentration 12,5% was 11,7 mm. Minimum kill concentration ethyl acetate fraction against P. aeruginosa ATCC 27853 of 12,5%. The chemical contents of the most effective ethyl acetate fraction were tested by TLC. The result of identification showed that ethyl acetate fraction positively contained alkaloid, flavonoids, saponin and tannin. Keywords : Yodium leaf (J. multifida), P. aeruginosa ATCC 27853, n-hexana, ethyl acetate and water fraction.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Yodium leaf (J. multifida), P. aeruginosa ATCC 27853, n-hexana, ethyl acetate and water fraction. |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Farmasi > Prodi S1 Farmasi |
| Depositing User: | Herera Gadys USB |
| Date Deposited: | 25 Feb 2019 03:40 |
| Last Modified: | 25 Feb 2019 03:40 |
| URI: | http://repo.setiabudi.ac.id/id/eprint/1239 |
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