UJI AKTIVITAS ANTIOKSIDAN FRAKSI n-HEKSANA, FRAKSI ETIL ASETAT, DAN FRAKSI AIR EKSTRAK ETANOL DAUN WARU (Hibiscus tiliaceus L.) SERTA PENETAPAN KADAR FLAVONOID TOTAL

KHAMIDAH, Nur (2016) UJI AKTIVITAS ANTIOKSIDAN FRAKSI n-HEKSANA, FRAKSI ETIL ASETAT, DAN FRAKSI AIR EKSTRAK ETANOL DAUN WARU (Hibiscus tiliaceus L.) SERTA PENETAPAN KADAR FLAVONOID TOTAL. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Antioxidants are compounds which capable to remove the effects of free radicals. Free radicals are molecule that contain one or more unpaired electrons and highly reactive. Waru leaf contains saponin, polyphenol and flavonoid that can be used as an antioxidant. This study was aimed to determine the antioxidant activity of n-hexane, ethyl acetate and water fractions as well as ethanol extract of waru leaf, to determine the greatest antioxidant activity between n-hexane, ethyl acetate and water fractions as well as ethanol extract waru leaf, and to determine total flavonoid levels of fraction that has greatest antioxidant activity. Waru leaf (Hibiscus tiliaceus L.) was extracted using maceration method with ethanol 96%, followed by fractionation using n-hexane, and ethyl acetate solvents. Test of antioxidant activity to DPPH radical was done to n-hexane, ethyl acetate, water fractions as well as ethanol extract of waru leaf. Determination of total flavonoid levels was done to ethyl acetate fraction. The test results of antioxidant activity which expressed by IC 50 value to the n-hexane, ethyl acetate, water fractions and ethanol extract of waru leaf were ppm 483.8290, 152.9438 ppm, 363,0339 ppm and 257.8545 ppm, respectively. The greatest antioxidant activity was ethyl acetate fraction. The results of total flavonoids determination in ethyl acetate fraction was 25.3671 %. Keywords: waru leaf, antioxidant, n-hexane fraction, ethyl acetate fraction, water fraction, ethanol extract, total flavonoid

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	waru leaf, antioxidant, n-hexane fraction, ethyl acetate fraction, water fraction, ethanol extract, total flavonoid
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	01 Oct 2019 03:19
Last Modified:	01 Oct 2019 03:19
URI:	http://repo.setiabudi.ac.id/id/eprint/1435

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