IDENTIFIKASI FITOKIMIA FRAKSI-FRAKSI NONPOLAR EKSTRAK ETANOL BATANG BROTOWALI (Tinospora crispa L.) DAN KEMAMPUAN PENGHAMBATAN ENZIM ASETILKOLINESTERASE

Dewi, Citra (2014) IDENTIFIKASI FITOKIMIA FRAKSI-FRAKSI NONPOLAR EKSTRAK ETANOL BATANG BROTOWALI (Tinospora crispa L.) DAN KEMAMPUAN PENGHAMBATAN ENZIM ASETILKOLINESTERASE. Tesis thesis, Universitas Setia Budi Surakarta.

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Abstract

Acetylcholinesterase (AChE) is an enzyme that plays a role hidrolizes acetylcholine. Acetylcholine plays a role in memory and cognitive function. Brotowali had been shown to improve memory and cognitive function. This research aims to determine the IC50 value and chemical compounds from nonpolar fractions of brotowali ethanol extract that can inhibit the enzyme acetylcholinesterase in vitro. The method used in this study is the separation of chemical compounds by extraction and fractionation sokhletasi using Vacuum Liquid Chromatography (KCV) and analyzed by LC-MS and inhibition of the enzyme acetylcholinesterase testing using the Ellman method (colorimetric). The parameters observed with IC50 values than the control Neostigmine. The results showed that the IC50 values, respectively fraction I and fraction II is at 384.942 and 369.088 µg/ml while the IC50 value of the ethanol extract and fractions III brotowali can not be determined (˃400 µg/ml). Neostigmine as positive control showed IC50 value of ˂15,625 µg/ml. Fraction II was the most active fraction and LC-MS results obtained by five compounds that play a role in the inhibition of the enzyme acetylcholinesterase that stigmasterol, N-trans feruloiltiramin, borapetol, sicloeucalenon, and octasanol . Key words : Tinospora crispa (L.), Extract Ethanol, Nonpolar Fraction, Inhibition of Acetylcholinesterase.

Item Type:	Thesis (Tesis)
Uncontrolled Keywords:	Tinospora crispa (L.), Extract Ethanol, Nonpolar Fraction, Inhibition of Acetylcholinesterase.
Subjects:	Q Science > QD Chemistry R Medicine > R Medicine (General)
Divisions:	Fakultas Farmasi > Prodi S2 Farmasi
Depositing User:	magdalena kartika ningsih
Date Deposited:	20 Feb 2020 07:10
Last Modified:	20 Feb 2020 07:10
URI:	http://repo.setiabudi.ac.id/id/eprint/2201

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