UJI AKTIVITAS KOMBINASI FRAKSI ETIL ASETAT EKSTRAK ETANOL JAMUR TIRAM (Pleurotus ostreatus) DAN DOKSORUBISIN TERHADAP SEL T47D, SEL VERO DAN PROLIFERASI SEL LIMFOSIT SECARA IN VITRO

Torry, Grisye (2015) UJI AKTIVITAS KOMBINASI FRAKSI ETIL ASETAT EKSTRAK ETANOL JAMUR TIRAM (Pleurotus ostreatus) DAN DOKSORUBISIN TERHADAP SEL T47D, SEL VERO DAN PROLIFERASI SEL LIMFOSIT SECARA IN VITRO. Tesis thesis, Universitas Setia Budi Surakarta.

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Abstract

Considering the previous studies suggesting that oyster mushroom (Pleurotus ostreatus) has potential therapeutic and preventive effect on breast cancer and serves as immunomodulator agent, the objective of current research was to find out the activity proliferation of lymphocyte cell and cytotoxic effect of ethyl acetic fraction of oyster mushroom ethanol extract (FEJT) in vitro. This study also aimed to find out the effect of FEJT combined with doxorubicin on T47D cell, Vero cell and identification the compound contributing activity to the examination. The extraction of oyster mushroom was conducted using maceration method with ethanol 96% and then fractionation with Thin Liquid Chromatography (TLC). FEJT was tested for its effect on lymphocyte cell, T47D cell and Vero cell using MTT method with varying concentrations 50 µg/mL, 100 µg/mL, 200 µg/mL, 400 µg/mL, 800 µg/mL, 1000 µg/mL. The data obtained was analyzed using SPSS 17. The test results showed that FEJT activity can stimulate lymphocyte proliferation. Combined FEJT (701,27 μg/mL) and doxorubicin (0.005 μg/mL) had the highest cytotoxic effect on T47D cells and decrease the cytotoxic effects of doxorubicin against Vero cells. FEJT fractions containing steroids, alkaloids and flavonoids. Keywords: Oyster mushroom (Pleurotus ostreatus), lymphocyte, T47D cell, Vero cell.

Item Type:	Thesis (Tesis)
Uncontrolled Keywords:	Oyster mushroom (Pleurotus ostreatus), lymphocyte, T47D cell, Vero cell.
Subjects:	Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S2 Farmasi
Depositing User:	magdalena kartika ningsih
Date Deposited:	20 Feb 2020 07:27
Last Modified:	20 Feb 2020 07:27
URI:	http://repo.setiabudi.ac.id/id/eprint/2329

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