ANALISIS PEWARNA RHODAMIN B DALAM ARUM MANIS SECARA KROMATOGRAFI LAPIS TIPIS DAN SPEKTROFOTOMETRI UV-VIS DI DAERAH SUKOHARJO DAN SURAKARTA

Pamungkas, Retno Putri (2014) ANALISIS PEWARNA RHODAMIN B DALAM ARUM MANIS SECARA KROMATOGRAFI LAPIS TIPIS DAN SPEKTROFOTOMETRI UV-VIS DI DAERAH SUKOHARJO DAN SURAKARTA. Karya Tulis Ilmiah thesis, Universitas Setia Budi Surakarta.

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Abstract

Rhodamine B is a synthetic dye that has a function as a textile dye. Rhodamine B is often misused its function as a coloring agent in food though it was clear that Rhodamine B is prohibited for food uses by Regulation of the Minister of Health of Republic Indonesia No. 239/Menkes/Per/ V/85. This study aims to determine the presence of Rhodamine B on Arum Manis and know how levels of Rhodamine B in it . The research was conducted by using the withdrawal color method in the wool for qualitative test purpose. Extraction with eter p.a followed by purification using HCl 0,1 N then followed by UV-Vis spectrophotometry in 555 nm wavelength. Extract readings calculated by linear regression equations and statistical tests. The results showed that from the three samples studied contained one positive sample containing Rhodamine B, seen from the results of the TLC which showed Rf sample that nearly same as the standard. In reading with UV-Vis spectrophotometry generating uptake and after calculated using the linear regression equation and statistical tests was obtained Rhodamine B concentration of 0.02%. Key words: Rhodamine B, Arum Manis, TLC, UV-Vis Spectrophotometry.

Item Type: Thesis (Karya Tulis Ilmiah)
Uncontrolled Keywords: Rhodamine B, Arum Manis, TLC, UV-Vis Spectrophotometry.
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Farmasi > Prodi D3 Analis Farmasi dan Makanan
Depositing User: magdalena kartika ningsih
Date Deposited: 21 Feb 2020 01:28
Last Modified: 21 Feb 2020 01:28
URI: http://repo.setiabudi.ac.id/id/eprint/2416

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