EFEK PENGHAMBATAN ANAFILAKSIS KUTAN AKTIF KOMBINASI EKSTRAK BIJI JINTAN HITAM (Nigella sativa L.) DAN HERBA SAMBILOTO (Andrographis paniculata N.) PADA TIKUS YANG DIINDUKSI OVALBUMIN

Palupi, Dian Arsanti (2014) EFEK PENGHAMBATAN ANAFILAKSIS KUTAN AKTIF KOMBINASI EKSTRAK BIJI JINTAN HITAM (Nigella sativa L.) DAN HERBA SAMBILOTO (Andrographis paniculata N.) PADA TIKUS YANG DIINDUKSI OVALBUMIN. Tesis thesis, Universitas Setia Budi Surakarta.

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Abstract

Black cumin seeds (Nigella sativa L.) and herbaceous bitter/sambiloto (Andrographis paniculata N.) have been potentially studied to inhibit mast cell degranulation. This study aimed was to determine the inhibitory activity of active cutaneous anaphylaxis of 96% ethanol extract of black cumin seeds (BCS) and bitter herbs (BH) combination on ovalbumin-induced rats. Animal tests were grouped into 10 groups (@4 rats). Group I-II were treated with BSC or BH only. Group III-VII were treated orally with the combination BCS:BH. Group VIII by 0,5% of CMC-Na orally , Group IX was treated sub-cutaneously by 2% of cromolin –Na, and well as the normal group (X). The treatment of extract were given through of the research. Group of I-X were sensitized subcutaneously twice (1x per week) in the back of the rats by ovalbumin. At the second week of testing, all animals were injected intravenously by 1,5% of Evans blue. For supporting the pharmacological data, the histopathology the skin tissue by toluidine blue staining, was done. The data obtain was analyzed statistically. The results of statistical tests cromolyn sodium group, extracts of black cumin and bitter dose of 30 mg, 120 mg; (15:60) mg; (22,5:30) mg; (7,5:90) mg; (7,5:60) mg; (22,5:90) mg / kg body weight resulted in a significant difference to the negative control, and produce the percent inhibition of active cutaneous anaphylaxis respectively 14.30 ± 6.15 *; 11.79 ± 5.74 *; 34.81 ± 4.85 *; 27.02 ± 4.66 *; 21.05 ± 5.84 *; 23.84 ± 4.73 *; 38.52 ± 4.71 *, these results are supported by observations of histopathological tissue showed a very strong correlation between the number of intact mast cells with active cutaneous anaphylaxis percent inhibition. Keywords: Nigella sativa L. Andrographis paniculata N., active cutaneous anaphylaxis, ovalbumin, mast cells.

Item Type:	Thesis (Tesis)
Uncontrolled Keywords:	Nigella sativa L. Andrographis paniculata N., active cutaneous anaphylaxis, ovalbumin, mast cells.
Subjects:	Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S2 Farmasi
Depositing User:	magdalena kartika ningsih
Date Deposited:	21 Feb 2020 02:55
Last Modified:	21 Feb 2020 02:55
URI:	http://repo.setiabudi.ac.id/id/eprint/2538

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