UJI AKTIVITAS ANTIBAKTERI EKSTRAK, FRAKSI n-HEKSANA,ETIL ASETAT DAN AIR DAUN BIDURI (Calotropis gigantea) TERHADAP Staphylococcus aureus ATCC 25923 DAN Escherichia coli ATCC 25922

Hartadi, Daniel Khrisna Dwi (2019) UJI AKTIVITAS ANTIBAKTERI EKSTRAK, FRAKSI n-HEKSANA,ETIL ASETAT DAN AIR DAUN BIDURI (Calotropis gigantea) TERHADAP Staphylococcus aureus ATCC 25923 DAN Escherichia coli ATCC 25922. Skripsi thesis, Universitas Setia Budi.

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Abstract

Biduri plant (Calotropis gigantea) is a plant that contains chemical compounds flavonoids, tannins and saponins which are efficacious as antibacterial to infections caused by bacteria. The aim of this study was to determine antibacterial activity of extract, n-hexane, ethyl acetate and water fractions of biduri leaves against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, the most active fraction, MIC and MBC value. Biduri leaves were extracted with maceration method and fractionated using n-hexane, ethyl acetat and water. Antibacterial activities of extract and fractions were tested by diffusion method concentration 50%, 25% and 12,5% and dilution method concentration 50%, 25%, 12,5%, 6,25%, 3,13%, 1,57%, 0,79%, 0,40%, 0,20% dan 0,10%. The data obtained were carried out statistical analysis using two way ANOVA. The results showed that the extracts and leaf fractions of biduri had antibacterial activity against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The ethyl acetate fraction of biduri leaves concentration of 50% had the most active antibacterial activity, Minimum Bactericidal Concentration (MBC) is 6,25% against Staphylococcus aureus ATCC 25923 and 12,5% against Escherichia coli ATCC 25922. Key words: biduri leaves, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, antibacterial.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: biduri leaves, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, antibacterial.
Subjects: Q Science > QK Botany
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Tifany Nur Arfiana
Date Deposited: 01 Nov 2019 03:34
Last Modified: 01 Nov 2019 03:34
URI: http://repo.setiabudi.ac.id/id/eprint/3588

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