UJI AKTIVITAS ANTIBAKTERI EKSTRAK, FRAKSI n-HEKSANA,ETIL ASETAT DAN AIR DAUN SENDUDUK BULU [Clidemia hirta (L.) D. Don] TERHADAP Staphylococcus aureus ATCC 25923 DAN Escherichia coli ATCC 25922

Arvian, Dias Wahyu (2019) UJI AKTIVITAS ANTIBAKTERI EKSTRAK, FRAKSI n-HEKSANA,ETIL ASETAT DAN AIR DAUN SENDUDUK BULU [Clidemia hirta (L.) D. Don] TERHADAP Staphylococcus aureus ATCC 25923 DAN Escherichia coli ATCC 25922. Skripsi thesis, Universitas Setia Budi.

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Abstract

Senduduk bulu plants [Clidemia hirta (L). D. Don] is efficacious as a medicine for wounds, bacterial infections and diarrhea. The aim of this study was to determine antibacterial activity of extract, n-hexane, ethyl acetate and water fractions of senduduk bulu leaves against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, the most active fraction, MIC and MBC value. Senduduk bulu leaves were extracted with ethanol 96% and fractionated using n-hexane, ethyl acetat and water. Antibacterial activities of extract and fractions were tested by diffusion method concentration 20%, 10% and 5% and dilution method concentration 20%, 10%, 5%, 2.5%, 1.25%, 0.625%, 0.312%, 0.156%, 0.078% and 0.039%. The data obtained were carried out statistical analysis using two way ANOVA followed by the Tukey test. The results showed that the extracts and leaf fractions of senduduk bulu had antibacterial activity against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The ethyl acetate fraction of senduduk bulu leaves concentration of 20% had the most active antibacterial activity, Minimum Bactericidal Concentration (MBC) of 0.625% against Staphylococcus aureus ATCC 25923 and 1.25% against Escherichia coli ATCC 25922. Key words: Senduduk bulu leave, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, antibacterial.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Senduduk bulu leave, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, antibacterial.
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	01 Nov 2019 03:36
Last Modified:	01 Nov 2019 03:36
URI:	http://repo.setiabudi.ac.id/id/eprint/3621

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