UJI AKTIVITAS SITOTOKSIK EKSTRAK DAN FRAKSI TANAMAN KELADI TIKUS (Typhonium flagelliforme (Lodd) BI) TERHADAP SEL KANKER PAYUDARA T47D

Agustina, Eka Wardanandri (2019) UJI AKTIVITAS SITOTOKSIK EKSTRAK DAN FRAKSI TANAMAN KELADI TIKUS (Typhonium flagelliforme (Lodd) BI) TERHADAP SEL KANKER PAYUDARA T47D. Skripsi thesis, Universitas Setia Budi.

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Abstract

Rodent taro (Typhonium flagelliforme (L.)) has the ability to inhibit cancer cell growth, suppress the detrimental effects of chemotherapy, antiviral and anti-bacterial. This study aims to determine the cytotoxic activity of extracts and fractions of rodent tuber on T47D breast cancer cells and find out the selectivity index for vero cells. Rodent taro plants were extracted by maceration method with 96% ethanol and fractionated using ethyl acetate and n-hexane. Cytotoxic activity tests were carried out using the MTT (Microculture Tetrazolium Technique) method with a 500 concentration series; 250; 125; 62.5; 31.2; 15.6; 7.8 µg / mL, positive control uses a concentration series 1; 0.5; 0.25; 0.125; 0.0625; 0.03125; 0.016 µg / mL. To calculate IC50, a linear regression equation was made between log concentration vs % viability. Rodent taro extract had IC50 value of 160.605 µg / mL, ethyl acetate fraction of 99.776 µg / mL, n-hexane fraction of 47.476 µg / mL showed potent activity on T47D breast cancer cells. The water fraction has IC50 223.132 µg / mL value and is less potent for T47D breast cancer cells. The selectivity index of ethanol extract was 5.919, ethyl acetate fraction was 3.172, n-hexane fraction was 3.715, and the water fraction was 5.052 which showed that the extract and fraction had good selectivity for normal cells. Keywords: rodent taro plants, T47D cells, cytotoxic, selectivity index

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: rodent taro plants, T47D cells, cytotoxic, selectivity index
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Tifany Nur Arfiana
Date Deposited: 01 Nov 2019 03:38
Last Modified: 01 Nov 2019 03:38
URI: http://repo.setiabudi.ac.id/id/eprint/3636

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