UJI AKTIVITAS SITOTOKSIK EKSTRAK DAN FRAKSI RIMPANG JAHE MERAH (Zingiber officinale Rosc Var. Rubrum) TERHADAP SEL KANKER HATI HepG2

Sari, Emy Rizki Nardhinta (2019) UJI AKTIVITAS SITOTOKSIK EKSTRAK DAN FRAKSI RIMPANG JAHE MERAH (Zingiber officinale Rosc Var. Rubrum) TERHADAP SEL KANKER HATI HepG2. Skripsi thesis, Universitas Setia Budi.

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Abstract

Liver cancer is the fourth leading cause of death that often occurs in men rather than women. The red ginger rhizome (Zingiber officinale Rosc Var. Rubrum) is empirically used to treat anti-cancer. This study aims to determine the cytotoxic activity of extracts, ethyl acetate fraction, n-hexane fraction and water fraction on HepG2 liver cancer cells and to determine the selectivity index for vero cells, The red ginger rhizome was extracted using the remaseration method with 96% ethanol and fractionated using ethyl acetate, n-hexane and water. Cytotoxic activity test of extracts and ethyl, n-hexane and red ginger rhizome fractions were carried out using the MTT method with a concentration series of 500; 250; 125; 62.5; 31.2; 15.6; 7.8125 µg / ml while in the positive control using the concentration series namely 2; 1; 0.5; 0.25; 0.125; 0.0625; 0.03125 µg / ml. The test results of cytotoxic activity of red ginger rhizome extract had an IC50 value of 86,635 µg / ml, IC50 ethyl acetate fraction: 51,627 µg / ml and IC50 n-hexan fraction: 39,250 µg/ml (<100 µg/ ml) showed moderate cytotoxic activity against HepG2 liver cancer cells. IC50 value in water fraction: 132,62 µg / ml. The IC50 doxorubicin:2.29µg / ml. The selectivity index of the extract, ethyl acetate fraction and water gave a maximum effect on normal cells, while doxorubicin caused cytotoxicity in normal cells. . Keywords: cytotoxic, HepG2 cell, red ginger rhizome, selectivity index

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	cytotoxic, HepG2 cell, red ginger rhizome, selectivity index
Subjects:	Q Science > QK Botany
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	01 Nov 2019 03:38
Last Modified:	01 Nov 2019 03:38
URI:	http://repo.setiabudi.ac.id/id/eprint/3638

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