PENAPISAN BAKTERI PENGHASIL ENZIM SUPEROKSIDA DISMUTASE (SOD) DARI TANAH HUTAN MANGROVE MARON EDUPARK SEMARANG

Wulandari, Lestari (2019) PENAPISAN BAKTERI PENGHASIL ENZIM SUPEROKSIDA DISMUTASE (SOD) DARI TANAH HUTAN MANGROVE MARON EDUPARK SEMARANG. Skripsi thesis, Universitas Setia Budi.

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Abstract

Superoskida Dismutase (SOD) enzyme is known as an antioxidant that can minimize tissue damage due to free radicals. SOD can be produced from bacteria on mangrove forest land. This study aims to isolate and find out the isolates that produce SOD activity, and measure the highest SOD activity and identify the isolates that are the highest SOD producers. The samples used were 5 isolates that have been based on a different character macroscopically. Fifth bacterial isolates tested SOD activity with the WST-1 assay kit and produce a percentage inhibition value reduction. The highest SOD activity was shown with a large percentage of inhibition, then identified. The percent inhibition results showed that all bacterial isolates had SOD activity where the highest SOD activity was THM1 with an inhibitory percent value of 74.51%. THM1 samples were tested conventionally with Gram positive results. Molecular test with PCR 16S rDNA showed the identity of THM1 isolates namely Bacillus altitudinis with 99% homology. Based on the search with the UniProt program it was proven that Cu-Zn SOD type SOD was registered. Keywords: free radicals, bacteria, mangrove, SOD

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	free radicals, bacteria, mangrove, SOD
Subjects:	Q Science > QK Botany
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	05 Nov 2019 04:18
Last Modified:	05 Nov 2019 04:18
URI:	http://repo.setiabudi.ac.id/id/eprint/3720

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