Cahyaningrum, Ninda Putri (2019) ISOLASI, UJI AKTIVITAS, DAN KARAKTERISASI ENZIM PROTEASE DARI BAKTERI Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Salmonella typhi ATCC 13311, Serratia marcescens ATCC 8100, DAN Staphylococcus aureus ATCC 25923. Skripsi thesis, Universitas Setia Budi.
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Abstract
Protease enzyme is a biocatalyst for the reaction of protein breakdown into oligopeptides or amino acids. Protease plays an important role in many industries. Protease enzymes can be obtained from bacteria either naturally or through genetic engineering. This study aimed to determine the activity and characterization of protease enzymes from Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Salmonella typhi ATCC 13311, Serratia marcescens ATCC 8100, and Staphylococcus aureus ATCC 25923. The research was conducted in several stages, namely identification of bacteria which included observations of morphology, Gram staining, and biochemical testing. Bacterial fermentation and test activities for the crude extract of protease enzymes used the diffusion method in the Skim Milk Agar media. Determination of enzyme activity was tested based on the influence of temperature and pH. The results showed that the five bacteria used in this study were able to produce protease enzymes. The largest protease activity originated from P. aeruginosa ATCC 27853 with a clear zone diameter of 15.62 mm. Protease characteristics of P. aeruginosa ATCC 27853 have an optimum temperature and pH of 30oC and pH of 8. Keywords: Characteristics, proteolytic bacteria, protease enzyme
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Characteristics, proteolytic bacteria, protease enzyme |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Fakultas Farmasi > Prodi S1 Farmasi |
| Depositing User: | Tifany Nur Arfiana |
| Date Deposited: | 12 Nov 2019 07:35 |
| Last Modified: | 12 Nov 2019 07:35 |
| URI: | http://repo.setiabudi.ac.id/id/eprint/3771 |
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