UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, KLOROFORM, DAN AIR EKSTRAK ETANOL 70% DAUN KARANDAS (Carissa carandas L.) TERHADAP Escherichia coli ATCC 25922

Lorensa, Nova Maya (2019) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, KLOROFORM, DAN AIR EKSTRAK ETANOL 70% DAUN KARANDAS (Carissa carandas L.) TERHADAP Escherichia coli ATCC 25922. Skripsi thesis, Universitas Setia Budi.

	Text 1. INTISARI DAN ABSTRAK.pdf Download (158kB)
	Text 2. FORM PUBLIKASI.pdf Download (374kB)
	Text 3. COVER - BAB I.pdf Download (804kB)
	Text 4. BAB II.pdf Download (550kB)
	Text 5. BAB III.pdf Download (515kB)
	Text 6. BAB IV.pdf Download (595kB)
	Text 7. BAB V - LAMPIRAN.pdf Download (3MB)

Abstract

Karandas leaves (Carissa carandas L.) from the Apocynaceae family are plants that have been widely used by the community in the treatment of diarrhea. Karandas leaves contain alkaloid compounds, flavonoids, saponins, tannins, steroids and triterpenoids. The aim of this study was to determine the antibacterial activity of n-hexan, chloroform, and ethanol extract of carandas leaves, as well as to determine the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericide Concentration (MBC) of the most active fractions of carandas against Escherichia coli ATCC 25922. Karandas leaves extracted was obtained by maceration with 70% ethanol, then fractionated with n-hexane, chloroform, and water solvents. Ethanol extract, n-hexane, chloroform, and water fraction were tested for antibacterial activity using disc diffusion method with concentrations of 50%, 25%, and 12,5%. The most active fraction obtained from the diffusion method was then followed by a dilution method with a concentration of 25%; 12,5%; 6,25%; 3,12%; 1,56%; 0,78%; 0,39%; and 0,19%. Statistical analysis using one way ANOVA, identification of the chemical content of carandas leaves using a tube test and Thin Layer Chromatography (TLC). The test results showed that 70% ethanol extract, n-hexane fraction, chloroform, and water had antibacterial activity. The greatest inhibitory power is the chloroform fraction with an average of 13,67 mm at a concentration of 50%. The results of this study showed that the Minimum Killer Concentration of chloroform fraction was 6,25%. Keywords: Escherichia coli ATCC 25922, Carissa carandas, diffusion, dilution

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Escherichia coli ATCC 25922, Carissa carandas, diffusion, dilution
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	12 Nov 2019 07:35
Last Modified:	12 Nov 2019 07:35
URI:	http://repo.setiabudi.ac.id/id/eprint/3776

Actions (login required)

View Item