SKRINING DAN IDENTIFIKASI BAKTERI PENGHASIL ENZIM SUPEROKSIDA DISMUTASE TAHAN PANAS DENGAN METODE PCR 16S rDNA

Rini, Lindya Aprila Agung Fistya (2020) SKRINING DAN IDENTIFIKASI BAKTERI PENGHASIL ENZIM SUPEROKSIDA DISMUTASE TAHAN PANAS DENGAN METODE PCR 16S rDNA. Skripsi thesis, Universitas Setia Budi.

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Abstract

Superoxide Dismutase (SOD) enzyme is known as an antioxidant that can minimize tissue damage due to free radicals. It is produced from thermophile bacteria in heat-resistant area. This study aimed to isolate isolates that produced SOD, determine SOD activity, and identify SOD-producing isolates with the highest activity using 16 rDNA molecular PCR methods. Samples were taken from the Sikidang Dieng crater area, of which they were undertaken in three different craters with each one sampling point. The mud samples were taken from the crater surface and the sediment laid at the bottom of the crater. The samples were isolated and three bacterial isolates were selected based on their morphological differences. Data of SOD activity test and bacterial species characterization were obtained from literature studies, where the test was administered using spectrophotometry by inducing superoxide radicals produced from the reaction between xanthine and xanthine oxidase The bacterial isolates were tested for SOD activity based on SOD's ability to inhibit Nitroblue Tetrazolium reduction ( NBT) by superoxide. The results of the inhibition from the literature study showed that all thermophile bacterial isolates had SOD activity where the highest SOD activity was Thermothrix sp. with an inhibition value of 15%. Molecular identification of SOD-producing bacteria was carried out on the bacteria originating from Manikaran hot spring named Anoxybacillus gonensis. Based on a search with the database program GenBank proved that the bacteria was registered in SOD type Cu/znSOD. Keywords: SOD, free radicals, thermophile bacteria, hot spring, PCR 16rDNA

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	SOD, free radicals, thermophile bacteria, hot spring, PCR 16rDNA
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	29 Mar 2022 07:54
Last Modified:	29 Mar 2022 07:54
URI:	http://repo.setiabudi.ac.id/id/eprint/4686

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