MANEAK, Irene Elisabeth (2018) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI n-HEKSANA, ETIL ASETAT, SERTA AIR DARI DAUN JAMBU AIR (Syzygium aqueum) TERHADAP PERTUMBUHAN Escherichia coli ATCC 25922. Skripsi thesis, Universitas Setia Budi Surakarta.
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SKRIPSI Irene Elisabeth Maneak ( 20144238 A )..pdf Download (2MB) |
Abstract
Syzygium aqueum the family of Myrtaceae is a plant that has been widely used by the public in the field of medicine. Guava leaves (Syzygium aqueum) contain flavonoid, fenolik and tanin. The aim of the experiment was to determine the antibacteria activity of ethanol extract, fraction of n-hexane, ethyl acetate and water from guava leaves and to know Minimum Inhibition Concentration (MIC) and Minimum Bactericide Concentration (MBC) active fraction from guava leaves against the growth of Escherichia coli ATCC 25922. Guava leaves extracted by maceration with 70% ethanol, then fractionated with n-hexane, ethyl acetate, and water solvent. After that, tested for antibacteria activity using disk diffusion method with multiple concentrations. The most active fraction obtained from the diffusion method is then followed by the dilution method with concentrations of 50%, 25%, 12,5%, 6,25%, 3,12%, 1,56%, 0,78%, 0,39%, 0,19% and 0,095%. The result of antibacterial activity from 70% ethanol extract, fraction of nhexane, ethyl acetate and water shows the presence of inhibitory power by the clear area around the disk. The largest mean inhibitory diameter is the ethyl acetate fraction with the average was 28 mm at concentration of 50%. The ethyl acetate fraction of guava leaves had the most effective antibacterial activity compared with 70% ethanol extract, fraction of n-hexane, and water. The result of the experiment with dilution method of ethyl acetate fraction showed that Minimum Bactericide Concentrations of ethyl acetate fraction was 12,5%. Keywords : Syzygium aqueum, Escherichia coli ATCC 25922, diffusion, dilution.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Syzygium aqueum, Escherichia coli ATCC 25922, diffusion, dilution. |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Farmasi > Prodi S1 Farmasi |
| Depositing User: | Tifany Nur Arfiana |
| Date Deposited: | 23 Feb 2019 02:40 |
| Last Modified: | 23 Feb 2019 02:40 |
| URI: | http://repo.setiabudi.ac.id/id/eprint/478 |
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