POTENSI EKSTRAK ENZIM DAUN MANGGA KOPYOR (Mangifera indica L.) SEBAGAI AGEN FIBRINOLITIK SECARA IN VITRO

Baihaqi, Handika Izza (2020) POTENSI EKSTRAK ENZIM DAUN MANGGA KOPYOR (Mangifera indica L.) SEBAGAI AGEN FIBRINOLITIK SECARA IN VITRO. Skripsi thesis, Universitas Setia Budi.

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Abstract

Fibrinolytic agents are used to degrade fibrin in blood clots so that the degradation products will become more soluble in the blood. Fibrinolytic enzymes can be produced from plants, one of which is the mango plant. This study aims to determine the potential of kopyor mango leaf enzyme extract (Mangifera indica L.) and determine the most effective concentration as fibrinolytic agent in vitro. The research began with the determination of kopyor mango (Mangifera indica L.), material collection, extraction of kopyor mango leaf fibrinolytic enzymes by centrifugation method, purification of kopyor mango leaf enzyme extract by depositing ammonium sulfate (first enzyme purification pellet) followed by TCA or acetone precipitation, methanol washing and phenol extraction (second enzyme purification pellet), protein purity measurement, Lowry method protein assay and fibrinolytic potential test using fibrin plate method in vitro. Kopyor mango leaf enzyme extract has potential as fibrinolytic agent in vitro characterized by the formation of a clear zone on fibrin media. Effective concentration, that is 80%, has optimal potential as fibrinolytic agent. 80% (37.3mm) supernatant sample, 80% first enzyme purification pellet (45.51mm), and second enzyme purification pellet (45.77mm). The sample of the second enzyme purification pellet at concentration of 80% has optimal potential as in vitro fibrinolytic agent. Keywords: Mangifera indica L., fibrinolytic, fibrin plate, Lowry

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Mangifera indica L., fibrinolytic, fibrin plate, Lowry
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	09 Jun 2022 03:31
Last Modified:	09 Jun 2022 03:31
URI:	http://repo.setiabudi.ac.id/id/eprint/4830

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