KARAKTERISASI ENKAPSULASI EKSTRAK KASAR ENZIM SOD BUAH TOMAT (Solanum lycopersicum L.) MENGGUNAKAN METODE GELASI IONIK DENGAN VARIASI KONSENTRASI KITOSAN & TRIPOLIFOSFAT

Prameswari, Meinanda Dyah (2021) KARAKTERISASI ENKAPSULASI EKSTRAK KASAR ENZIM SOD BUAH TOMAT (Solanum lycopersicum L.) MENGGUNAKAN METODE GELASI IONIK DENGAN VARIASI KONSENTRASI KITOSAN & TRIPOLIFOSFAT. Skripsi thesis, Universitas Setia Budi.

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Abstract

Continuous exposure to free radicals can result in oxidative stress. Tomato fruit (Solanum lycopersicum L.) contains the antioxidant enzyme Superoxide Dismutase (SOD) which can neutralize free radicals in the body. The low level of enzyme stability makes it difficult in the formulation process and affects the effect of the therapy to be given. The nanoencapsulation system in which the SOD enzyme is wrapped at a nanometer scale is expected to protect this enzyme from degradation and deliver it to the target site. The superoxide dismutase enzyme from tomatoes is obtained by extraction, centrifugation and precipitation using 90% Ammonium sulfate. The protein content of the crude extract was measured using the Lowry method with Bovine Serum Albumin (BSA) as a standard. Superoxide dismutase activity in crude extract was determined using the WST-1 assay kit method. The nanoencapsulation was made using the ionic gelation method using 3 variations of the concentration of chitosan and tripolyphosphate, namely F1 (0.1%: 0.3%); F2 (0.2%: 0.2%); and F3 (0.3%: 0.1%). The success of the encapsulation process was observed from the percentage of efficiency encapsulation, while the nanoparticles characterization tested included particle size, polydispersity index and zeta potential. The results obtained were crude extract of the SOD enzyme of tomato fruit containing 0.0369 ± 0.000049 mg / mL protein with a superoxide dismutase activity of 89.22%. The efficiency encapsulation values for each formula were F1 65.58%; F2 79.89%; and F3 72.72%. F2 is the formula with the highest efficiency encapsulation compared to the other two formulas. Nanoparticle characterization against F2 gave the result that the particle size was 711.83 ± 66.50nm, with a polydispersity index of 0.289 ± 0.123 single peak and a zeta potential value of + 14.9 ± 9.59 mV. Key words: superoxide dismutase, Solanum lycopersicum L., nanoencapsulation, ionic gelation

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	superoxide dismutase, Solanum lycopersicum L., nanoencapsulation, ionic gelation
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	13 Jun 2022 06:54
Last Modified:	13 Jun 2022 06:54
URI:	http://repo.setiabudi.ac.id/id/eprint/4840

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