Fu, Armando Chan Chi (2021) ISOLASI KITOSAN DARI CANGKANG BEKICOT (Achatina fulica) DAN UJI AKTIVITASNYA TERHADAP BAKTERI Shigella dysenteriae ATCC 9361 DENGAN METODE DIFUSI SERTA KAJIAN PUSTAKA TENTANG AKTIVITAS KITOSAN TERHADAP BAKTERI GRAM NEGATIF. Skripsi thesis, Universitas Setia Budi.
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Abstract
Snail shells (Achatina fulica) are believed to contain chitosan compounds which are derivatives of chitin compounds and can be obtained after going through the isolation process. The purpose of this study was to determine the chitosan content of chitosan isolates from snail shells with FTIR, antibacterial potential and effective concentration against Shigella dysenteriae bacteria and the antibacterial potential of chitosan isolates from natural ingredients against Gram negative bacteria Shigella dysenteriae, Escherichia coli, and Pseudomonas aeruginosa. This research was conducted by isolating chitosan in snail shells through the deproteination stage using 3% NaOH at a ratio of 1:10, demineralization of 1 N HCl at a ratio of 1:12, and deacetylation of 60% NaOH at a ratio of 1:15. Chitosan isolated from snail shell chitin was continued with qualitative analysis by FTIR. Chitosan isolated from snail shell chitin was then tested for antibacterial activity by well diffusion method against Shigella dysenteriae bacteria at concentrations of 10000 ppm, 5000 ppm, and 2500 ppm. Literature study on Gram negative bacteria was carried out to support the results of the antibacterial activity of chitosan against Shigella dysenteriae bacteria. The results showed that the chitosan compound group contained in the snail shell chitin isolate after being determined by FTIR with the main group bending NH possessed by the amine at a wave number of 1643.4 cm-1. Chitosan isolated from snail shell chitin has antibacterial activity with the most effective concentration at 10000 ppm against Shigella dysenteriae bacteria. Chitosan isolated from natural ingredients has antibacterial activity against Gram negative bacteria Shigella dysenteriae, Escherichia coli, and Pseudomonas aeruginosa. Keywords : Chitosan, isolation, Shigella dysenteriae, Gram negative.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Chitosan, isolation, Shigella dysenteriae, Gram negative. |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Farmasi > Prodi S1 Farmasi |
| Depositing User: | Tifany Nur Arfiana |
| Date Deposited: | 21 Jul 2022 03:57 |
| Last Modified: | 21 Jul 2022 03:57 |
| URI: | http://repo.setiabudi.ac.id/id/eprint/4938 |
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