UJI AKTIVITAS FIBRINOLITIK DARI EKSTRAK KASAR ENZIM BAKTERI Bacillus altitudinis MENGGUNAKAN METODE Plate Fibrin

Susilowati, Putri Asih (2021) UJI AKTIVITAS FIBRINOLITIK DARI EKSTRAK KASAR ENZIM BAKTERI Bacillus altitudinis MENGGUNAKAN METODE Plate Fibrin. Skripsi thesis, Universitas Setia Budi.

	Text 1. ABSTRAK.pdf Download (91kB)
	Text 4. BAB I.pdf Download (489kB)
	Text 5. BAB II.pdf Restricted to Repository staff only Download (259kB) | Request a copy
	Text 6. BAB III.pdf Restricted to Repository staff only Download (384kB) | Request a copy
	Text 7. BAB IV.pdf Restricted to Repository staff only Download (754kB) | Request a copy
	Text 8. BAB V.pdf Restricted to Repository staff only Download (85kB) | Request a copy
	Text 9. DAFTAR PUSTAKA.pdf Download (314kB)
	Text 10. LAMPIRAN.pdf Download (519kB)
	Text 2. Form Pernyataan Publikasi_Putri Asih Susilowati.pdf Download (106kB)
	Text 3. Surat Keterangan Plagiasi_Putri Asih Susilowati.pdf Download (29kB)

Abstract

Fibrinolytic enzymes are proteases capable of degrading fibrin. One of the fibrinolytic enzymes is derived from Bacillus bacteria. Mangrove forest soil contained Bacillus altitudinis bacteria after isolation. The purpose of this study was to determine the fibrinolytic activity of the crude extract of the enzyme Bacillus altitudinis in lysing fibrin from mangrove forest soil. This study begins with identification of the gene encoding fibrinolytic with NCBI then identification of Bacillus altitudinis bacteria on blood agar media, Gram staining, endospores, catalase test and coagulase test. Production of bacterial protein crude extract was carried out by centrifugation followed by cell breakdown by sonication. Measurement of protein levels using the Bradford method and the fibrinolytic activity test using the fibrin plate method. Concentrations of 20, 40, and 80% crude extract of the enzyme Bacillus altitudinis, nattokinase as a positive control, while aquadest as a negative control. The data obtained were statistically analyzed using One Way Anova. The bacterium Bacillus altitudinis contains the AprE gene encoding fibrinolytic. Bacillus altitudinis bacteria have beta hemolysis activity, are Gram positive, form endospores, have catalase and coagulase enzymes. Enzyme extraction yields 4.5 mL. The total protein content of the crude extract of the fibrinolytic enzyme Bacillus altitudinis was 22,908 mg/mL. The best concentration of crude extract of Bacillus altitudinis enzyme in lysing fibrin was 80% concentration with a clear zone of 7.92 mm. Keywords : Bacillus altitudinis¸ fibrinolytic, plate fibrin¸ nattokinase, bradford

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Bacillus altitudinis¸ fibrinolytic, plate fibrin¸ nattokinase, bradford
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	05 Aug 2022 01:37
Last Modified:	05 Aug 2022 01:37
URI:	http://repo.setiabudi.ac.id/id/eprint/5027

Actions (login required)

View Item