UJI AKTIVITAS EKSTRAK KASAR ENZIM FIBRINOLITIK DARI BAKTERI Bacillus cereus YANG DIISOLASI DARI AIR HUTAN MANGROVE MARON EDUPARK SEMARANG SECARA IN VITRO

Asnan, Rizky Bimantara Hanafi (2021) UJI AKTIVITAS EKSTRAK KASAR ENZIM FIBRINOLITIK DARI BAKTERI Bacillus cereus YANG DIISOLASI DARI AIR HUTAN MANGROVE MARON EDUPARK SEMARANG SECARA IN VITRO. Skripsi thesis, Universitas Setia Budi.

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Abstract

Fibrinolytic enzymes are enzymes that work by degrading fibrin in blood clots. Fibrinoytic enzymes can be obtained from mangrove forest water. Mangrove ecosystems are source of various microbes that are able to produce useful enzymes and molecules. Fibrinolytic enzymes are produced by one of them by the bacterium B.cereus. This study aims to determine the activity of the fibrinolytic enzyme B.cereus in lysing blood clots in vitro. The study began with identification of genes using NCBI software and bacterial morphology on BAP media, Gram staining, endospore staining and testing of catalase and coagulase. Isolation of B.cereus fibrinolytic enzyme was carried out by enzyme extraction. The protein content of the enzyme extract was determined using the Bradford method and the in vitro fibrinolytic activity test was carried out with fibrin media. The clear zone data obtained were analyzed using the SPSS application. The results of the identification of the B.cereus bacterial gene in the NCBI software were registered as the AprE gene. The results of the identification of Gram staining and endospores of B.cereus are Gram positive bacteria and have endospores. Macroscopic identification of B.cereus showed positive results in the -hemolysis category. The results of the catalase and coagulase tests showed positive results. The protein content of the B.cereus fibrinolytic enzyme extract obtained was 19.63 mg/mL. The results showed that the crude extract of the bacterial fibrinolytic enzyme B.cereus was able to degrade fibrin. The most effective concentration of various concentrations of 20, 40, and 80% crude extract of B.cereus fibrinolytic enzyme as a fibrinolytic agent in vitro is 80% concentration. Keywords: Bacillus cereus; Bradford; Fibrinolytic; Fibrin Plate; Nattokinase

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Bacillus cereus; Bradford; Fibrinolytic; Fibrin Plate; Nattokinase
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	05 Aug 2022 01:36
Last Modified:	05 Aug 2022 01:36
URI:	http://repo.setiabudi.ac.id/id/eprint/5037

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