UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK DAUN TEH HIJAU (Camellia sinensis L) DAN EKSTRAK BIJI PEPAYA (Carica papaya L) TERHADAP BAKTERI Escherichia coli ATCC 25922

Apriani, Aulia (2021) UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK DAUN TEH HIJAU (Camellia sinensis L) DAN EKSTRAK BIJI PEPAYA (Carica papaya L) TERHADAP BAKTERI Escherichia coli ATCC 25922. Skripsi thesis, Universitas Setia Budi.

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Abstract

Green tea leaves (Camellia Sinensis L) have antibacterial activity, compounds containing flavanoids, tannins, saponins, and alkaloids. Papaya seeds (Carica Papaya L) papaya seed plant has antibacterial activity, compounds contained alkaloids, saponins, and flavanoids. This study was to find out the antibacterial activity of the combination of green tea leaf extract (Camellia Sinensis L) and papaya seeds (Carica Papaya L), with a comparison of the active effects of the combination of extracts against escherichia coli bacteria ATCC 25992. Extract green tea leaves and papaya seeds using the maceration method with a 96% ethanol solvent. Single extracts were tested using a series of green tea concentrations of 100%,80%, 40%, 20%, 10%, 5%, 2.5%, 1.25%, 0.62%, 0.31%, and papaya seed extract 40%, 20%, 10%, 5%, 2.5%, 1.25%, 0.62%, 0.31%, 0.15%, 0.07%. Extract is tested in combination with disc diffusion method with comparison of green tea leaf extract and papaya seeds (1:1) 10% green tea extract: papaya seed extract 20%, (1:2) green tea extract 10%: papaya seed extract 40%, and (2:1) green tea extract 20%: papaya seed extract 20%. The results were analyzed using SPSS statistic analysis version 21. Combination testing is done to determine the pattern of the combination. The results of the study that the dilution test with the results of Minimal Kill Concentration (KBM) green tea leaves with a concentration of 10% and papaya seeds with a concentration of 20% have been able to kill the growth of bacteria. Results of a combination diffusion test of green tea leaf extract and papaya seeds using a combination (2:1) It has antibacterial activity against escherichia coli bacteria ATCC 25992 with an average diameter of 27.29 mn. The results of the combination interaction pattern test using the most active combination (2:1) obtained results that are patterned synergistically. . Keywords: Camellia Sinensis, Carica Papaya L,Escherichia coli ATCC 25992, dilution, diffusion,combination

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Camellia Sinensis, Carica Papaya L,Escherichia coli ATCC 25992, dilution, diffusion,combination
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Tifany Nur Arfiana
Date Deposited: 29 Sep 2022 04:38
Last Modified: 29 Sep 2022 04:38
URI: http://repo.setiabudi.ac.id/id/eprint/5264

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