UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, ETIL ASETAT DAN FRAKSI AIR DARI EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) TERHADAP BAKTERI Escherichia coli

Maninten, Manik (2021) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, ETIL ASETAT DAN FRAKSI AIR DARI EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) TERHADAP BAKTERI Escherichia coli. Skripsi thesis, Universitas Setia Budi.

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Abstract

Cherry plant (Muntingia calabura L.) contains compounds such of flavonoids, tannins, saponins and steroids which have antibacterial properties. This study aimed to test the antibacterial activity and fractions of n-hexane, ethyl acetate, and water using ethanol extract of cherry leaf (Muntigia calabura L.) against Escherichia coli and to determine the most active fraction and the value of Minimum Inhibitory Concentration (MIC) and Minimum Kill Concentration (KBM) against Escherichia coli. Cherry leaves were extracted by maceration method using 96% ethanol and then fractioned using n-hexane, ethyl acetate and water as solvents. Testing of antibacterial activity using the diffusion method with a concentration of 20%, 10%, 5% and dilution method with a concentration of 20%; 10%; 5%; 2,5%; 1,25%; 0,625%; 0,312%; 0,156%; 0,078%; 0,039%. The data obtained were statistically analyzed by one way ANOVA. The results showed that the ethanol extract, n-hexane, ethyl acetate, and water fractions from cherry leaves had antibacterial activity against Escherichia coli. The most active fraction obtained was the ethyl acetate fraction with a concentration of 20% with an average value of inhibition 19,0 mm. The Minimum Kill Concentration (KBM) of the ethyl acetate fraction of cherry leaves against Escherichia coli is 5% and and the Minimum Inhibitory Concentration (MIC) of the ethyl acetate fraction of cherry leaves was not visible. Keywords : Antibacterial, Cherry leaf, diffusion, dilution, Escherichia coli

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Antibacterial, Cherry leaf, diffusion, dilution, Escherichia coli
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	10 Oct 2022 07:31
Last Modified:	10 Oct 2022 07:31
URI:	http://repo.setiabudi.ac.id/id/eprint/5350

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