ISOLASI BAKTERI-BAKTERI ENDOFIT PADA DAUN TANAMAN SUKUN (Artocarpus altilis) YANG BERPOTENSI MENGHAMBAT PERTUMBUHAN Streptococcus mutans ATCC 25175

Wardiani, Martha Aulia (2021) ISOLASI BAKTERI-BAKTERI ENDOFIT PADA DAUN TANAMAN SUKUN (Artocarpus altilis) YANG BERPOTENSI MENGHAMBAT PERTUMBUHAN Streptococcus mutans ATCC 25175. Skripsi thesis, Universitas Setia Budi.

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Abstract

Endophytic bacteria are bacteria that live in plant tissues and are non-pathogenic. Endophytic bacteria produce bioactive compounds whose characteristics are similar to their host. The bioactive compounds were obtained by isolating them from endophytic bacteria. Breadfruit leaves have antibacterial properties. The aim of the study was to obtain isolates of endophytic bacteria from breadfruit leaves which showed antibacterial activity against Sterptococcous mutans. The research was conducted by isolating group A endophytic bacteria from young leaves, still fresh, not wilted and free of pests and diseases; group B from leaves that are quite old, fresh, not wilted, free of pests and diseases. The isolate was fermented for 74 hours. The separation between the supernatant and biomass using a 5,000 rpm centrifuge for 40 minutes. The resulting supernatant was tested for antibacterial activity using the disc diffusion method, negative control in the form of discs immersed in sterile BHI and positive control using amoxicillin. The clear area indicates the inhibition of the growth of microorganisms by antimicrobial agents on the surface of the agar medium. The results showed that the leaves of the breadfruit plant (Artocarpus altilis) could be isolated from endophytic bacteria and the isolates obtained had the potential to inhibit the growth of Streptococcus mutans ATCC 25175. Group A had an average diameter of 8.74 mm, the antibacterial power was in the medium category and group B had a diameter of 8.74 mm. an average of 11.04 mm antibacterial power in the strong category. Keywords : Endophytic microbes, breadfruit plant leaves, Streptococcus mutans, antibacterial.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Endophytic microbes, breadfruit plant leaves, Streptococcus mutans, antibacterial.
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	10 Oct 2022 07:31
Last Modified:	10 Oct 2022 07:31
URI:	http://repo.setiabudi.ac.id/id/eprint/5358

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