PENETAPAN KADAR VITAMIN C PADA KACANG BUNCIS (Phaseolus vulgaris L.) SEGAR dan REBUS SECARA SPEKTROFOTOMETRI UV

Andarwati, Anisa Septy (2017) PENETAPAN KADAR VITAMIN C PADA KACANG BUNCIS (Phaseolus vulgaris L.) SEGAR dan REBUS SECARA SPEKTROFOTOMETRI UV. Karya Tulis Ilmiah thesis, Universitas Setia Budi Surakarta.

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Abstract

Vitamin C is one of the nutrients which plays a role as the antioxidant and is effective in overcoming free radicals that can destroy cells or tissue. The source of vitamin C is generally found in oranges, vegetables that have green leaves like green beans. This study aims to discover the presence of vitamin C in fresh green beans and boiled green beans and also to determine the amount of vitamin C in fresh green beans and boiled green beans by using the spectrophotometric UV. The vitamin C testing in fresh and boiled green beans was started by qualitative analysis, then was continued by quantitative analysis by using Spectrophotometric UV and the analysis method used was the standard basic curve method. The steps of the study was done through making of basic solution, determining of maximum wave with the range of 240nm-280nm with the interval of 5 nm, determining the operating time with the interval of 1 minute for 20 minutes, and determining the concentration variation of the basic solution of vitamin C which are 4,72µg/ml; 7,08µg/ml; 9,44µg/ml; 11,8µg/ml; 14,16µg/ml observed in the maximum λ. The treatment on the samples which are fresh green beans and boiled green peas in 100 , after boiled, blended, and sentrifuged which is then analyzed by using Spectrophotometric UV. Based on the findings in the laboratorium, it was known that the fresh green beans and boiled green beans contain vitamin C and by using the spectrophotometric UV it was obtained that the amount of vitamin C in fresh green beans was 0.20% and in the boiled green beans was 0.16% Keywords : Vitamin C, Fresh and Boiled Green Beans, Spectrophotometric UV

Item Type: Thesis (Karya Tulis Ilmiah)
Uncontrolled Keywords: Vitamin C, Fresh and Boiled Green Beans, Spectrophotometric UV
Subjects: R Medicine > R Medicine (General)
Divisions: Fakultas Farmasi > Prodi D3 Farmasi
Depositing User: Rohma Tri Wardani
Date Deposited: 23 Feb 2019 02:48
Last Modified: 23 Feb 2019 02:48
URI: http://repo.setiabudi.ac.id/id/eprint/541

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