UJI AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE (SOD) DALAM EKSTRAK TEMU HITAM (Curcuma aeruginosa Roxb.) DENGAN METODE WATER SOLUBLE TETRAZOLIUM SALT-1 (WST-1)

Novarini, Tita (2022) UJI AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE (SOD) DALAM EKSTRAK TEMU HITAM (Curcuma aeruginosa Roxb.) DENGAN METODE WATER SOLUBLE TETRAZOLIUM SALT-1 (WST-1). Skripsi thesis, Universitas Setia Budi.

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Abstract

Reactive oxygen species (ROS) are free radicals that play an important role in physiological processes in the body, but if the amount is too high, it can cause oxidative stress. ROS can be eliminated in the presence of endogenous antioxidants such as SOD enzymes. SOD is an enzyme that catalyzes superoxide anion radicals into oxygen derivatives, but not into free radicals. One of the herbs with SOD activity is temu hitam (Curcuma aeruginosa Roxb). This study aims to determine the total protein content and the percentage inhibition of crude temu hitam SOD extract at an ammonium sulfate precipitation concentration of 25, 50, 75, 100% and determine the percentage of inhibition of the optimal concentration of ammonium sulfate precipitation. This research begins with the determination of plants and the taking of temu hitam samples. The extraction of the SOD enzyme was performed by adding PBS and centrifuging. The purification of the SOD enzyme was carried out by the ammonium sulfate precipitation method. Determination of the total protein content by the Lowry method. Measurement of SOD activity was performed by measuring the percentage inhibition value using the Superoxide Dismutase Activity Assay Kit WST-1. The results showed that the total protein content of the crude extract and the precipitation of ammonium sulfate were 25, 50, 75 and 100% 0.909, respectively; 0.639; 0.710; 0.752; and 0.944 mg/ mL. Then the percent inhibition values were 76,720, respectively; 23,810; 65,079; 70,370; and 83.069%, the optimum concentration being a concentration of 100%. Kata kunci : Curcuma aeruginosa Roxb., SOD, WST-1.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Curcuma aeruginosa Roxb., SOD, WST-1.
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	30 Nov 2022 04:18
Last Modified:	30 Nov 2022 04:18
URI:	http://repo.setiabudi.ac.id/id/eprint/5632

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