AKTIVITAS EKSTRAK ENZIM BUAH MENTIMUN (Cucumis sativus L.) SEBAGAI AGEN FIBRINOLITIK DENGAN METODE CLOT LYSIS SECARA IN VITRO

Napitupulu, Verdy Mangarah Samaludin (2022) AKTIVITAS EKSTRAK ENZIM BUAH MENTIMUN (Cucumis sativus L.) SEBAGAI AGEN FIBRINOLITIK DENGAN METODE CLOT LYSIS SECARA IN VITRO. Skripsi thesis, Universitas Setia Budi.

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Abstract

Acute myocardial infarction (IMA) is a disease caused by a blockage of a blood clot (thrombus) in the coronary arteries that causes blood flow and oxygen distribution to be blocked. The Ministry of Health in 2018 stated that as many as 7,200,000 patients died from IMA worldwide. Fibrinolytic agents play an enzymatic degradation of fibrin and serve to free the vessels from blood clots. Fibrinolytic enzymes can be produced from plants, one of which is derived from cucumber plants. The study aimed to find out the potency of cucumber enzyme extract (Cucumis sativus L.) and find out the most effective concentrations as fibrinolytic agents in vitro. The research began with the determination of the cucumber fruit plant (Cucumis sativus L.), the retrieval of ingredients, the extraction of cucumber enzymes, the purification of cucumber enzyme extract with the deposition of ammonium sulfate 60% (1st enzyme purification pellet), purification of cucumber enzymes with TCA or acetone (2nd enzyme purification pellet), determination of Lowry method protein levels and fibrinolytic potential test with clot lysis method in vitro. The concentration of extracts will be made in three variations namely 20, 40, and 80%. Cucumber enzyme extracts in protein levels testing by Lowry method get results are 1,260 μg/mL for 1st enzyme purification pellets; 2.305 μg/mL for 2nd purification pellets; and 0.831 μg/mL for coarse enzymes while effective concentrations, i.e. 80% have optimal potential as fibrinolytic agents. 1st enzyme purification pellet samples 80% (47%), 2nd enzyme purification pellets (81%), and coarse extract samples (82%). Samples of the 2nd enzyme purification pellet at a concentration of 80% have optimal potential as a fibrinolytic agent in vitro. Keywords: Cucumis sativus L., fibrinolytic agent, IMA, clot lysis method

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Cucumis sativus L., fibrinolytic agent, IMA, clot lysis method
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	30 Nov 2022 02:45
Last Modified:	30 Nov 2022 02:45
URI:	http://repo.setiabudi.ac.id/id/eprint/5639

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