UJI AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE (SOD) EKSTRAK DAUN SALAM (Syzygium polyanthum) DENGAN METODE WATER SOLUBLE TETRAZOLIUM SALT-1 (WST-1)

Sari, Fitriana Novita (2023) UJI AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE (SOD) EKSTRAK DAUN SALAM (Syzygium polyanthum) DENGAN METODE WATER SOLUBLE TETRAZOLIUM SALT-1 (WST-1). Skripsi thesis, Universitas Setia Budi.

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Abstract

Antioxidants are substances that can destroy free radicals. One of the antioxidant enzymes is SOD. One of the plants that have SOD activity is bay leaf because it has high enough SOD activity so it can ward off free radicals that damage cells in the human body causing several diseases. The aim of the research was to determine the total protein content and purification of ammonium sulfate and the percent inhibition of the crude extract of the superoxide dismutase enzyme of bay leaves with ammonium sulfate concentrations of 25, 50, 75% and the optimum inhibition value of the amonium sulfate concentration. This study used bay leaves (Syzygium polyanthum) extracted with phosphate buffer and centrifuged. SOD enzyme purification with ammonium sulfate concentrations of 25, 50, 75%. Measuring protein levels with the Lowry method and SOD activity test using the WST-1 method to calculate the percent inhibition. The results were statistically analyzed using one way ANOVA. The results showed that the SOD extraction obtained 175 ml of crude extract. The weight of ammonium sulfate refining pellets with concentrations of 25, 50, and 75% were 0.1252; 0.1860; 1.4687. The total protein content of the crude extract and the amonium sulfate precipitation of 25, 50, and 75% respectively were 9.377; 6.356; 8,501; and 11.6 mg/mL. Then the percentage inhibition values were 73.133; 34,327; 65,671; and 80.590% with the optimum concentration being 75%. Keywords: Syzygium polyanthum, free radicals, SOD, WST-1

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Syzygium polyanthum, free radicals, SOD, WST-1
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	05 Apr 2023 02:56
Last Modified:	05 Apr 2023 02:56
URI:	http://repo.setiabudi.ac.id/id/eprint/5782

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