UJI AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE (SOD) EKSTRAK DAUN JAMBU BIJI (Psidium guajava L.) DENGAN METODE WATER SOLUBLE TETRAZOLIUM SALT-1 (WST-1)

Mandasari, Mitha Oktavia (2022) UJI AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE (SOD) EKSTRAK DAUN JAMBU BIJI (Psidium guajava L.) DENGAN METODE WATER SOLUBLE TETRAZOLIUM SALT-1 (WST-1). Skripsi thesis, Universitas Setia Budi.

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Abstract

Antioxidants are substances that can prevent cell damage caused by free radicals. Antioxidant enzymes include superoxide dismutase, glutathione, and catalase. SOD can protect cells against free radicals that cause several diseases. One of the plants with SOD activity is the guava plant. The purpose of this research is to ascertain the superoxide dismutase enzyme activity in guava leaf extrac with ammonium sulfate concentrations of 25%, 50%, dan 75% using the Water Soluble Tetrazolium Salt-1 method. This study used guava leaves which were extracted by adding phosphate buffer and centrifuging. Ammonium sulfate is used to purify the SOD enzyme at concentrations of 25, 50, and 75%. The Lowry method was used to assess protein content next, and the WST-1 method was used to measure SOD activity and determine the percent inhibition and analyzed using one way ANOVA. The results showed that guava leaf extract had SOD enzyme activity. Total protein content of the crude extract guava leaves and purified ammonium sulfate concentrations of 25, 50 and 75% were 9.683 mg/mL; 6,958 mg/mL; 8,842mg/mL; and 11.269 mg/mL. The percent inhibition values were respectively 69.652%; 35,323%; 61.69% and 79.104% with the most optimum concentration is at a concentration of 75%. Keywords: Psidium guajava L., free radicals, antioxidants, SOD, WST-1

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Psidium guajava L., free radicals, antioxidants, SOD, WST-1
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	03 Jul 2023 04:26
Last Modified:	03 Jul 2023 04:26
URI:	http://repo.setiabudi.ac.id/id/eprint/5953

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