UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK ETANOL 70% KULIT NANAS (Ananas comosus L) DAN DAUN ALPUKAT (Persea americana Mill) TERHADAP BAKTERI Staphylococcus aureus

Wati, Rika Nur Rahma (2024) UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK ETANOL 70% KULIT NANAS (Ananas comosus L) DAN DAUN ALPUKAT (Persea americana Mill) TERHADAP BAKTERI Staphylococcus aureus. Skripsi thesis, Universitas Setia Budi.

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Abstract

RIKA NUR RAHMA WATI, 2023, ANTIBACTERI ACTIVITY TEST OF COMBINATION OF ETANOL 70% EXTRACT OF NANAS (Ananas comosus L) FRUIT AND ALPUKAT (Persea americana Mill) LEAVES AGAINST THE BACTERI Staphylococcus aureus, SKRIPSI, FAKULTAS FARMASI, UNIVERSITAS SETIA BUDI, SURAKARTA. Supervised by Dr. apt. Ismi Rahmawati, M.Si and apt. Ghani Nurfiana Fadma Sari, M.Farm. Pineapple fruit peel (Ananas comosus L) and avocado leaves (Persea americana Mill) contain alkaloid, flavonoid, tannin and saponin compounds. The content of these compounds has antibacterial activity. Previous research on ethanol extracts of pineapple peel and avocado leaves has antibacterial activity against Staphylococcus aureus. This study aims to determine the antibacterial activity of a combination of ethanol extracts of pineapple peel and avocado leaves against the growth of Staphylococcus aureus bacteria. Pineapple peel and avocado leaves were extracted using maceration method with 70% ethanol solvent, then phytochemical testing was conducted for the extracts. Determination of Minimum Kill Concentration of ethanol extracts of pineapple peel and avocado leaves was done by dilution method. The combination of pineapple peel and avocado leaf extracts was made in the ratio of (1:1), (1:2) and (2:1). Determination of antibacterial activity was done by disc diffusion method. The combination effect pattern was performed using the paper tape method, and the results were analyzed using SPSS statistics. The results showed that pineapple peel extract had a Minimum Kill Concentration value of 6.25% and avocado leaf had a Minimum Kill Concentration value of 12.5%. The combination of 1:1 has an inhibition of 18.9 ± 1.15. The 1:2 combination had an inhibition of 21.7 ± 0.35. The 2:1 combination had an inhibition of 24.1±0.72. The combination concentration of 2:1 is most effective in inhibiting the growth of S. aureus bacteria and provides a synergistic combination effect against S. aureus bacteria. RIKA NUR RAHMA WATI, 2023, UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK ETANOL 70% KULIT BUAH NANAS (Ananas comosus L) DAN DAUN ALPUKAT (Persea americana Mill) TERHADAP BAKTERI Staphylococcus aureus, SKRIPSI, FAKULTAS FARMASI, UNIVERSITAS SETIA BUDI, SURAKARTA. Dibimbing oleh Dr. apt. Ismi Rahmawati, M.Si dan apt. Ghani Nurfiana Fadma Sari, M.Farm. Kulit buah nanas (Ananas comosus L) dan daun alpukat (Persea americana Mill) mengandung senyawa flavonoid, alkaloid, tannin dan saponin. Kandungan senyawa tersebut memiliki aktivitas sebagai antibakteri. Penelitian sebelumnya ekstrak etanol kulit nanas dan daun alpukat memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri kombinasi ekstrak etanol kulit buah nanas dan daun alpukat terhadap pertumbuhan bakteri Staphylococcus aureus. Kulit buah nanas dan daun alpukat diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%, selanjutnya dilakukan pengujian fitokimia untuk ekstrak. Penentuan Konsentrasi Bunuh Minimum ekstrak etanol kulit buah nanas dan daun alpukat dilakukan dengan metode dilusi. Kombinasi ekstrak kulit nanas dan daun alpukat dibuat dengan perbandingan (1:1), (1:2) dan (2:1). Penentuan aktivitas antibakteri dilakukan dengan metode difusi cakram. Pola efek kombinasi dilakukan dengan menggunakan metode pita kertas, dan hasil tersebut dianalisis menggunakan statistic SPSS. Hasil penelitian menunjukkan bahwa ekstrak kulit buah nanas memiliki nilai Konsentrasi Bunuh Minimum 6,25% dan daun alpukat memiliki nilai Konsentrasi Bunuh Minimum 12,5%. Kombinasi 1:1 memiliki daya hambat 18,9±1,15. Kombinasi 1:2 memiliki daya hambat 21,7±0,35. Kombinasi 2:1 memiliki daya hambat 24,1±0,72. Konsentrasi kombinasi 2:1 paling efektif dalam menghambat pertumbuhan bakteri S. aureus dan memberikan efek kombinasi yang bersifat sinergis terhadap bakteri S. aureus.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Antibakteri, Staphylococcus aureus, Kulit buah nanas (Ananas comosus L), Daun alpukat (Persea americana Mill) Antibacterial, Staphylococcus aureus, Pineapple fruit peel (Ananas comosus L), Avocado leaf (Persea americana Mill)
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	USB
Date Deposited:	26 Jun 2024 07:38
Last Modified:	26 Jun 2024 07:38
URI:	http://repo.setiabudi.ac.id/id/eprint/6283

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