Thin Layer Chromatographic Separation of Amino Acids by Determining the Resistivity Factor Value (Pemisahan Kromatografi Lapis Tipis pada Asam Amino dengan Menentukan Nilai Faktor Retensi)

Dila Aulia, Hafizah and Sunardi, Sunardi (2024) Thin Layer Chromatographic Separation of Amino Acids by Determining the Resistivity Factor Value (Pemisahan Kromatografi Lapis Tipis pada Asam Amino dengan Menentukan Nilai Faktor Retensi). Jurnal Kimia dan Rekayasa, 5 (1). pp. 1-7. ISSN 2746-0886

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Abstract

Research has been conducted to separate amino acids using thin layer chromatography (CLT) by determining the retention factor (Rf) value. Amino acids are important components of proteins that have various functions in the body. KLT is a simple chromatographic method that is often used to separate and identify chemical components in a mixture. In this study, KLT was used to separate four types of amino acids, namely histidine, tryptophan, glycine, and alanine. The KLT procedure includes silica plate preparation, sample bottling, chromatogram formation by elution of various solvents, and determination of the Rf value of each sample. The results showed that each amino acid sample has a different Rf value due to differences in the polarity of each amino acid to the stationary phase of the silica plate and the solvent used. However, the Rf values obtained are not in accordance with the theory because there are errors in the preparation of silica plates.

Item Type:	Article
Uncontrolled Keywords:	Amino acids, chromatography, tryptophan, glycine, alanine, histidine.
Subjects:	Q Science > QD Chemistry
Divisions:	Fakultas Teknik > Prodi D3 Analis Kimia
Depositing User:	sunardi .
Date Deposited:	08 Aug 2024 04:21
Last Modified:	08 Aug 2024 04:21
URI:	http://repo.setiabudi.ac.id/id/eprint/6301

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