UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL FRAKSI n-HEKSAN, ETIL ASETAT DAN AIR DARI DAUN MURBEI (Morus alba L.) TERHADAP Escherichia coli ATCC 25922

SANUL, Kristina Erni (2018) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL FRAKSI n-HEKSAN, ETIL ASETAT DAN AIR DARI DAUN MURBEI (Morus alba L.) TERHADAP Escherichia coli ATCC 25922. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Mulberry (Morus alba L) leaf have chemical contents of flavonoid, alkaloid, polyphenol and terpenoids suspected of having antibacterial activity. This study is aims to determine the ability of ethanol extract, fraction of n-hexane, ethyl acetate and water from the Mulberry leaf (Morus alaba L.) against Escherichia coli ATCC 25922 Mulberry leaf extracted using method with 70% ethanol and then fractionated using n-hexane, ethyl acetate and water. Extract and the result of fractions tested the antibacterial using diffusion and dilution method. Diffusion method with a concentration of 50%, 25%, 12.5% and a positive control cotrimoxazole and negative control DMSO 3%. Dilution method using serial dilutions of 50%, 25 %, 12.5 %, 6.25 %, 3.12%. 1.56%, 0.78%, 0.39%, 0.1 %, 0.09, control positive and control negative The result of this study show that the fraction of ethyl acetate has the best antibacterial activity than the n-hexane fraction, water fraction and leaf ethanol extract of mulberry leaf. The best activity in ethyl acetate fraction of the ethanol extract of mulberry leaf at concentration of 50%, with inhibition 22.67 mm diffusion method. MKC fraction of ethyl acetate extract of bougainvillea leaf ethanol to kill Escherichia coli ATCC 25922 is a concentration of 3.12% dilution method. Keyword: Mulberry leaf, fractionation, antibacterial, Escherichia coli ATCC 25922

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Mulberry leaf, fractionation, antibacterial, Escherichia coli ATCC 25922
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	23 Feb 2019 03:22
Last Modified:	23 Feb 2019 03:22
URI:	http://repo.setiabudi.ac.id/id/eprint/676

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