UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, FRAKSI ETIL ASETAT, DAN FRAKSI AIR DARI EKSTRAK ETANOL DAUN ALPUKAT (Persea americana Mill) TERHADAP Proteus mirabilis ATCC 10975

MAYORGA, Andreas Hiba (2018) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, FRAKSI ETIL ASETAT, DAN FRAKSI AIR DARI EKSTRAK ETANOL DAUN ALPUKAT (Persea americana Mill) TERHADAP Proteus mirabilis ATCC 10975. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Avocado plants are one of the plants that can overcome the infection caused by bacteria. Part of the avocado plants that can be used as medicines are avocado seeds, avocado meat, and avocado leaves. Avocado leaves contain alkaloids, flavonoids, and saponins. This study aims to test the antibacterial activity of n-hexane fraction, ethyl acetate fraction, and water fraction of ethanolic extract of avocado leaf (Persea americana Mill) against Proteus mirabilis ATCC 10975 bacterium. Avocado leaf powder was extracted by maseration method using 70% ethanol solvent. The extracts obtained were fractionated with n-hexane, ethyl acetate and water solvents. Testing of Proteus mirabillis ATCC 10975 bacteria using diffusion method to find out the most active fraction then continued with dilution method to know Minimum Fill Concentration active fraction. The concentration used in the diffusion method was 41,67% and in the dilution method was used 20, 83% concentration 10,41%; 5,20%; 2,6%; 1,3%; 0,65%; 0,32%; 0,16%; 0,08%; 0,04%. The results showed that the fraction of n-hexane, ethyl acetate fraction, and water fraction of ethanol extract of avocado leaves had antibacterial activity against Proteus mirabilis ATCC 10975 at concentration 41,67%. Mean inhibitory diameter of n-hexane fraction, ethyl acetate fraction, and water fraction was 19,11 mm; 27.11 mm; and 20,80 mm. The most active fraction of Proteus mirabilis ATCC 10975 is an ethyl acetate fraction with a Minumum Kill Concentration of 12.5%. Keywords : Alpukat leaves,n-heksan fraction, ethyl acetat fraction, and water fraction, antibacterial, Proteus mirabilis.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Alpukat leaves,n-heksan fraction, ethyl acetat fraction, and water fraction, antibacterial, Proteus mirabilis.
Subjects:	R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	22 Feb 2019 07:24
Last Modified:	22 Feb 2019 07:24
URI:	http://repo.setiabudi.ac.id/id/eprint/72

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