UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, ETIL ASETAT DAN FRAKSI AIR DARI EKSTRAK METANOLIK DAUN MANGGA KASTURI (Mangifera casturi Kosterm) TERHADAP Staphylococcus aureus ATCC 25923

Pradana, Andri Adi (2017) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA, ETIL ASETAT DAN FRAKSI AIR DARI EKSTRAK METANOLIK DAUN MANGGA KASTURI (Mangifera casturi Kosterm) TERHADAP Staphylococcus aureus ATCC 25923. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Mango kasturi is one of the endemic plants of Borneo island which is believed to be able to provide some of the benefits. Mango bark kasturi tested can provide antibacterial activity. The purpose of this research is to find out whether the extracts, the fraction of n-heksana, ethyl acetate, and water fraction from leaves of mango kasturi can provide antibacterial activity against bacterium S. aureus, and to find out the value of the MIC and MKC from the active fraction . Kasturi mango leave were extracted by maceration method using methanol solvent, then fractionaion using n-heksanae, ethyl acetate, and water. Testing of antibacterial activity using dilution and diffusion method. The diffusion method compares the extract, n-hexane fraction, ethyl acetate fraction, water fraction, DMSO, and antibiotics as a comparison. The dilution method was carried out with the most active fraction dilution series on the test tube, after which inoculation was performed. Data analysis of the inhibit zone diameter with using by ANOVA. The result of antibacterial activity test with 25% concentration of methanol extract and the three fraction by diffusion method gave successive inhibitory activity that is 17,7mm; 12,24 mm; 21,5mm and 12,92mm. The ethyl acetate fraction has a MIC at 1.56%. Keywords: Mango kasturi, Staphylococcus aureus, dilution, diffusion, maceration

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Mango kasturi, Staphylococcus aureus, dilution, diffusion, maceration
Subjects: R Medicine > R Medicine (General)
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Rohma Tri Wardani
Date Deposited: 23 Feb 2019 04:22
Last Modified: 23 Feb 2019 04:22
URI: http://repo.setiabudi.ac.id/id/eprint/887

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