Kusumawati, Maria (2017) PENGARUH KOMBINASI ZAT PENGATUR TUMBUH (ZPT) BAP DAN 2,4-D TERHADAP PEMBENTUKAN KALUS SERTA IDENTIFIKASI SENYAWA LINALOOL DAN α-PINENE PADA KALUS ZODIA (Evodia suaveolens Scheff). Skripsi thesis, Universitas Setia Budi Surakarta.
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Abstract
Zodia leaves (Evodia suaveolens Scheff.) contains linalool and α-pinene compounds that serve as mosquito repellent. A long time to growth and the difficult culture zodia so needed plant propagation methods (tissue culture plants). The success of tissue culture is mainly caused by the presence of nutrient needs in the medium. The purposes of this research are to determine the effect of a combination of plant growth regulator (PGR) Benzylaminopurin (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) on the formation of calluses and identification of linalool and α-pinene compounds on calluses zodia (E. suaveolens Scheff.) Zodia leaves explant have grown on MS medium half strong with combined ratio of growth regulators BAP : 2,4-D 0:2 ppm; 0,5:1,5 ppm ; 1:1 ppm; 1,5:0,5 ppm ; 2:0 ppm. Zodia leaves calluses were harvested and extracted using n-hexane. The filtrate of n-hexane were analyzed by gas chromatography. The addition of PGR BAP and 2,4-D on MS medium half strong are to accelerate the growth and formation of calluses. Consentration 1 : 1 ppm is the best and fastest concentrationin the growth and formation of calluses. Zodia calluses are not containing α-pinene and linalool compounds. Keywords : zodia leaves explants, Murashige Skoog (MS) Medium half strong, BAP, 2,4-D, gas chromatography.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Keywords : zodia leaves explants, Murashige Skoog (MS) Medium half strong, BAP, 2,4-D, gas chromatography. |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Farmasi > Prodi S1 Farmasi |
| Depositing User: | Herera Gadys USB |
| Date Deposited: | 23 Feb 2019 04:23 |
| Last Modified: | 23 Feb 2019 04:23 |
| URI: | http://repo.setiabudi.ac.id/id/eprint/900 |
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