Sari, Bella Yunita Kartika (2021) PENETAPAN KADAR α-TOKOFEROL DALAM MINYAK ZAITUN (Olea europaea L.) DENGAN PELARUT ASETON SECARA KROMATOGRAFI CAIR KINERJA TINGGI. Skripsi thesis, Universitas Setia Budi.
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Abstract
Vitamin E also known as α-tocopherol, is an antioxidant present in olive oil. Because α-tocopherol is non-polar and fat soluble, it necessitates the use of a non-polar solvent to extract α-tocopherol molecules. To acquire the levels of α- tocopherol in Extra Virgin Olive Oil (EVOO), this study needed to create a solvent capable of producing the highest levels of α-tocopherol in a substance. The specific gravity and refractive index of olive oil samples were determined before HPLC analysis. The maximum wavelength was determined using a UV-VIS analyzer and different comparisons were carried out using HPLC derived from the values of tR, N, HETP, and TF at flow rates of 0.8 mL/minute, 1 mL/minute, and 1.5 mL/minute. The technique is then validated, which includes linearity, LOD, and other factors. The results showed that the optimum conditions for HPLC were a maximum wavelength of 286 nm, a flow rate of 1 mL/minute and an injection volume of 10 μL. The level of-tocopherol obtained in Extra Virgin Olive Oil (EVOO) olive oil is 0.145 ppm with a correlation coefficient (r) of 0.998, percent recovery value of 99.77 percent, and percent KV of 1.53 percent. From the results of the validation parameters carried out on the olive oil sample. Keywords : Zaitun oil, α-tocopherol, acetone, HPLC.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Zaitun oil, α-tocopherol, acetone, HPLC. |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Farmasi > Prodi S1 Farmasi |
| Depositing User: | Tifany Nur Arfiana |
| Date Deposited: | 21 Jul 2022 03:58 |
| Last Modified: | 21 Jul 2022 03:58 |
| URI: | http://repo.setiabudi.ac.id/id/eprint/4945 |
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