SURYANI, Suryani (2018) UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN, ETIL ASETAT, DAN AIR DARI EKSTRAK ETANOL DAUN SINGKONG (Manihot esculenta Crantz) TERHADAP BAKTERI Escherichia coli ATCC 25922. Skripsi thesis, Universitas Setia Budi Surakarta.
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Abstract
Cassava leaves (Manihot esculenta Crantz) contains flavonoid, saponins, and tannins which is used as an antibacterials. The aim of this research is to know the antibacterial activity of n-hexane fraction, ethyl acetate, and water from ethanol extract of cassava leaves (Manihot esculenta Crantz) against Escherichia coli ATCC 25922 bacteria and to know the value of Minimum Inhibitor Concentration (MIC) and Minimum Bactericidal Concentration (MBC) the most active fraction of cassava leaves (Manihot esculenta Crantz) against Escherichia coli ATCC 25922. Cassava leaves were extracted by maceration method using 70% ethanol solvent. The extract obtained was fractionated with n-hexane, ethyl acetate, and water solvents. Testing of antibacterial activity using diffusion method with concentration 50%, 25%, 12,5% and dilution method with concentration 50%; 25%; 12.5%; 6.25%; 3.12%; 1.56%; 0.78%; 0.39%; 0.19%; 0.09%. The results showed that the fraction of n-hexane, ethyl acetate, and water from ethanol extract of cassava leaves had antibacterial activity against Escherichia coli ATCC 25922 bacteria. Ethyl acetate fraction is the most active fraction that is at concentration 50% with mean inhibitory zone diameter 19,66 mm. The dilution results show the value of Minimum Bactericidal Concentration (MBC) is 12.5%. Keywords: Cassava leaves (Manihot esculenta Crantz), Escherichia coli ATCC 25922, nhexane fraction, ethyl acetate fraction, water fraction, antibacterial .
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Cassava leaves (Manihot esculenta Crantz), Escherichia coli ATCC 25922, nhexane fraction, ethyl acetate fraction, water fraction, antibacterial . |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Farmasi > Prodi S1 Farmasi |
| Depositing User: | Tifany Nur Arfiana |
| Date Deposited: | 25 Feb 2019 03:35 |
| Last Modified: | 25 Feb 2019 03:35 |
| URI: | http://repo.setiabudi.ac.id/id/eprint/1194 |
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