ANALISIS ASAM LEMAK DALAM PERTUMBUHAN BAKTERI Salmonella typhi ATCC 13311 DENGAN MENGGUNAKAN METODE KROMATOGRAFI GAS

Karimah, Teti Insani (2017) ANALISIS ASAM LEMAK DALAM PERTUMBUHAN BAKTERI Salmonella typhi ATCC 13311 DENGAN MENGGUNAKAN METODE KROMATOGRAFI GAS. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Infection is a process of invasion and breeding of microorganism that occur in the tissues of the human body. One of the infectious agents is Salmonella typhi bacteria. Detecting infectious bacteria in the human body by conventional methods usually takes 24 hours after examination of symptoms infection. The examination was considered less effective. This study aims to find the most optimal incubation time of bacteria Salmonella typhi ATCC 13311 which can be identified its compound by gas chromatography method. Salmonella typhi ATCC 13311 bacteria were cultured in BSA media and incubated at each time variation there were 6 hours, 12 hours, 24 hours, 36 hours and 48 hours. The bacterial fatty acid derivatisation of Salmonella typhi ATCC 13311 used esterification method which yields fatty acid methyl ester. Samples of fatty acid methyl ester for bacteria Salmonella typhi ATCC 13311 the next analyzed by gas chromatography with the initial temperature conditions of column 120 ° C. Gas chromatography was able to analyzed the sample of fatty acid methyl ester of bacteria Salmonella typhi ATCC 13311 at incubation time less than 24 hours and the optimal time of analysis was 6 hours. Results of retention time on analyzed of fatty acid methyl ester samples of bacteria Salmonella typhi ATCC 13311 at incubation 6 hours = 3.895, 12 hours = 3.935, 24 hours = 3.737, 36 hours = 3.468, 48 hours = 3.335. Keywords : Bacteria, Salmonella typhi ATCC 13311, gas chromatography, fatty acid methyl ester

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Bacteria, Salmonella typhi ATCC 13311, gas chromatography, fatty acid methyl ester
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Prodi S1 Farmasi
Depositing User: Herera Gadys USB
Date Deposited: 25 Feb 2019 03:41
Last Modified: 25 Feb 2019 03:41
URI: http://repo.setiabudi.ac.id/id/eprint/1246

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