PENGARUH PEMBERIAN KOMBINASI ZAT PENGATUR TUMBUH 2,4-D DAN KINETIN TERHADAP KANDUNGAN STEVIOSIDA PADA KALUS DAUN STEVIA

Elviyah, Rini (2014) PENGARUH PEMBERIAN KOMBINASI ZAT PENGATUR TUMBUH 2,4-D DAN KINETIN TERHADAP KANDUNGAN STEVIOSIDA PADA KALUS DAUN STEVIA. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Stevia (Stevia rebaudiana Bertonii M.) is one of the natural sweetener sources wich from Paraguay. Stevia leaf contains steviosida, rebaudiosida A, rebaudiosida B, rebaudiosida C, rebaudiosida D, rebaudiosida E, steviolbiosida, dulkosida A. Growth regulating substances is one of the complementary components that media plays an important role preformance process of plant growth and development. Of growth regulators used in this study is a combination of 2,4-D and kinetin. This research aimed to know increasing of growth controller 2,4-D and kinetin that well to do kalus induction of stevia leaf and know steviosida level wich containing in TLC densitometry manner. The study was conducted with plant tissue culture method using New Phalaenopsis (NP) medium with the addition of 2,4-D and kinetin plant growth regulator, with various concentration of 2,4-D 0 ppm and kinetin 1 ppm, 2,4-D 0,25 ppm and kinetin 0,75 ppm, 2,4-D 0,5 ppm and kinetin 0,5 ppm, 2,4-D 0,75 ppm and kinetin 0,25 ppm, 2,4-D 1 ppm and kinetin 0 ppm. Evaluation of callus was conducted on the percentage of success of explants to form callus, induction time, and the average weight of callus. Analysis of plant origin and callus conducted qualitatively by thin layer chromatography using a mobile phase of chloroformetanol-water (15:10:1) stationary phase silica gel 60 F 254 and TLC densitometry quantitative analysis. The results of research on the origin of plants an Stevia leaf callus with the addition of various concentration of growth regulator 2,4-D and kinetin able to stimulate the formation of callus Stevia leaf. Steviosida highest rate earned on additional growth regulator substances concentration 2,4-D 0 ppm and kinetin 1 ppm. Steviosid level contained in the Stevia leaf callus adaptation steviosida higher than the level contained in the plant origin. Keywords : Steviosida, Stevia leaves callus, New Phalaenopsis (NP), 2,4-D and kinetin

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Steviosida, Stevia leaves callus, New Phalaenopsis (NP), 2,4-D and kinetin
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	20 Feb 2020 02:25
Last Modified:	20 Feb 2020 02:25
URI:	http://repo.setiabudi.ac.id/id/eprint/2753

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