Fakih, Imas Qodri Nur (2019) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70%, FRAKSI n-HEKSANA, ETIL ASETAT, DAN AIR DARI DAUN STEVIA (Stevia rebaudiana Bertonii M.) TERHADAP Streptococcus mutans ATCC 25175 SECARA in vitro. Skripsi thesis, Universitas Setia Budi.
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1. INTISARI DAN ABSTRAK.pdf Download (89kB) |
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2. FORM PUBLIKASI.pdf Download (444kB) |
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3. COVER - BAB I.pdf Download (928kB) |
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Abstract
Stevia leaves contain some chemical compounds serving as antibacterial agent: flavonoid, alkaloid, saponin, tannin, and steroid. This research aimed to find out the antibacterial activity of n-hexane, ethyl acetate, and water fractions, and ethanol 70% extracts of stevia leaves, to find out which one has most effective antibacterial activity, whether ethanol extract or three fractions (n-hexane, ethyl acetate, water) of stevia leaves, and to find out MIC and MBC produced by the most active ethanol 70% extract of stevia leaves on Streptococcus mutans ATCC 25175. The antibacterial activity test in this study was conducted using diffusion and dilution methods. The concentrations of extract and fraction in diffusion method were 10%, 20%, and 40%. The most active fraction concentrations in dilution method were 40%, 20%, 10%, 5%, 2.5%, 1.25%, 0.625%, 0.3125%, 0.1563%, and 0.0782%. The most active fraction of Stevia leaves is identified for its chemical content using thin layer chromatography method. Ethanol, n-hexane fraction, ethyl acetate, and water extracts of stevia leaves have antibacterial activity. Ethyl acetate fraction 40% is the most active one with inhibiting zone diameter of 16.33 mm. Minimum Killing Concentration value of ethyl acetate fraction on Streptococcus mutans ATCC 25175 is 5%. Keywords: stevia leaves, fraction, Minimum Killing Concentration, Streptococcus mutans ATCC 25175.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | stevia leaves, fraction, Minimum Killing Concentration, Streptococcus mutans ATCC 25175. |
| Subjects: | R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Fakultas Farmasi > Prodi S1 Farmasi |
| Depositing User: | Tifany Nur Arfiana |
| Date Deposited: | 04 Nov 2019 03:48 |
| Last Modified: | 04 Nov 2019 03:48 |
| URI: | http://repo.setiabudi.ac.id/id/eprint/3685 |
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