UJI AKTIVITAS SITOTOKSIK DAN EKSPRESIPROTEINBcl-2 dan p53 EKSTRAK DAN FRAKSI DAUN ROSEMARY (Rosmarinus officinalis) TERHADAPSEL KANKER PAYUDARA T47D

Christiana, Priscila Wahyu (2020) UJI AKTIVITAS SITOTOKSIK DAN EKSPRESIPROTEINBcl-2 dan p53 EKSTRAK DAN FRAKSI DAUN ROSEMARY (Rosmarinus officinalis) TERHADAPSEL KANKER PAYUDARA T47D. Tesis thesis, Universitas Setia Budi Surakarta.

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Abstract

Rosemary leaves (Rosmarinus officinalis) are often found with anticancer activity. This study aims to determine the cytotoxic activity of extracts and fractions of rosemary leaves against T47D breast cancer cells, determine the selectivity index of T47D cancer cells against Vero cells and to determine the protein expression of Bcl-2 and p53 genes. The extraction method used was sokhletation with methanol as a solvent. The resulting extract is then fractionated with a liquid-liquid partition. Cytotoxic test using the MTT method assay with T47D breast cancer cells against vero cells and the results were read absorbance with ELISA Reader. Its effect on the expression of Bcl-2 and p53 gene proteins was seen with immunocytochemistry. IC50 data analysis and protein expression using the Anova statistical test. The results showed extract and ethyl acetate fraction of rosemary leaves had a low cytotoxic effect >50 μg/ml ie with IC50 respectively 60.359 μg/ml and 58.433 μg / ml. N-heksan fraction had a moderate cytotoxic effect < 50μg / ml with IC50 value 34,801 μg/ml. The selectivity index of extracts and ethyl acetate fraction has a value 3.3. Rosemary leaf extract can increase the expression of p53 and can inhibit the expression of Bcl-2 at a concentration of 30.179 – 120.718 μg/mland the n-hexane fraction can increase the expression of p53 and can inhibit the expression of Bcl-2 at concentrations of 17.401- 69.602 μg/ml. Keywords: Rosemary leaf (Rosmarinus Officinalis), cytotoxic, expression of Bcl-2 and p53, T47D breast cancer cells.

Item Type:	Thesis (Tesis)
Uncontrolled Keywords:	Rosemary leaf (Rosmarinus Officinalis), cytotoxic, expression of Bcl-2 and p53, T47D breast cancer cells
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S2 Farmasi
Depositing User:	Mrs NOVI HANDAYANI
Date Deposited:	13 Feb 2020 03:11
Last Modified:	16 Sep 2020 08:17
URI:	http://repo.setiabudi.ac.id/id/eprint/4122

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