PENETAPAN KADAR VITAMIN B1 DAN VITAMIN C PADA MINUMAN SUSU UHT DENGAN METODE SPEKTROFOTOMETRI UV MULTIWAVELENGTH

Prastiyo, Dony Dwi (2020) PENETAPAN KADAR VITAMIN B1 DAN VITAMIN C PADA MINUMAN SUSU UHT DENGAN METODE SPEKTROFOTOMETRI UV MULTIWAVELENGTH. Karya Tulis Ilmiah thesis, Universitas Setia Budi.

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Abstract

Vitamin B1 and vitamin C are water-soluble vitamins that play a very important role in humans. This study aims to determine the levels of vitamin B1 (Thimain HCl) and vitamin C (Ascorbic Acid) in UHT milk by using spectrophotometry UV multiwavelangth method. This research was conducted using the Multiwavelagth method. The samples used were UHT milk samples labeled with brand A; B and C. Then performed the determination ofabsorption the maximum wavelength, operating time, the absorption spectrum of five wavelength points and the determination of the levels of vitamin B1 and vitamin C using UV spectrophotometry. The results showed that the maximum wavelength of vitamin B1 232 nm and vitamin C was 266 nm. Operating time for vitamin B1 0 minutes and vitamin C 11 minutes. The single validation obtained by vitamin B1 has an accuracy of 100.10%, a precision of 0.26%, and vitamin C with an accuracy of 100.18%, a precision of 0.53%. For the validation of the mixture, vitamin B1 has an accuracy of 100.72%, precision is 1.24%, and vitamin C has an accuracy of 99.77%, precision is 0.92%. Vitamin B1 levels in brand A UHT milk; B; and C where the level of vitamin B1 for each sample brand A is 0.000752%; brand B 0.00676%; and brand C 0.00575%, for vitamin C the level cannot be determined because the absorbance in the 266 nm area is very small causing the matrix calculation to give negative concentration results. Keywords: UHT milk, Vitamin B1, Vitamin C, Multiwavelaght, spectrophotometry UV

Item Type: Thesis (Karya Tulis Ilmiah)
Uncontrolled Keywords: UHT milk, Vitamin B1, Vitamin C, Multiwavelaght, spectrophotometry UV
Subjects: R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Fakultas Farmasi > Prodi D3 Analis Farmasi dan Makanan
Depositing User: Tifany Nur Arfiana
Date Deposited: 17 Mar 2022 07:54
Last Modified: 17 Mar 2022 07:54
URI: http://repo.setiabudi.ac.id/id/eprint/4548

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