ISOLASI, UJI AKTIVITAS DAN KONSENTRASI SUBSTRAT OPTIMUM EKSTRAK ENZIM AMILASE DAN PROTEASE DARI BAKTERI Bacillus altitudinis

Wulandari, Nurhaliza Ayu (2021) ISOLASI, UJI AKTIVITAS DAN KONSENTRASI SUBSTRAT OPTIMUM EKSTRAK ENZIM AMILASE DAN PROTEASE DARI BAKTERI Bacillus altitudinis. Skripsi thesis, Universitas Setia Budi.

	Text INTISARI DAN ABSTRAK_NURHALIZA AYU WULANDARI_23175289A.pdf Download (88kB)
	Text BAB 1_NURHALIZA AYU WULANDARI_23175289A.pdf Download (1MB)
	Text BAB 2_NURHALIZA AYU WULANDARI_23175289A.pdf Restricted to Repository staff only Download (553kB) | Request a copy
	Text BAB 3_NURHALIZA AYU WULANDARI_23175289A.pdf Restricted to Repository staff only Download (543kB) | Request a copy
	Text BAB 4_NURHALIZA AYU WULANDARI_23175289A.pdf Restricted to Repository staff only Download (808kB) | Request a copy
	Text BAB 5_NURHALIZA AYU WULANDARI_23175289A.pdf Restricted to Repository staff only Download (1MB) | Request a copy
	Text DAFTAR PUSTAKA_NURHALIZA AYU WULANDARI_23175289A.pdf Download (175kB)
	Text LAMPIRAN_NURHALIZA AYU WULANDARI_23175289A.pdf Download (867kB)
	Text Form pernyataan bebas plagiasi sesuai dengan ketentuan Universitas.pdf Download (360kB)
	Text Form pernyataan publikasi_NURHALIZA AYU WULANDARI.pdf Download (149kB)

Abstract

Protease and amylase enzymes have been widely used in the food, pharmaceutical, and other chemical industries. Bacillus altitudinis is known to produce amylase and protease enzymes. The main factor affecting enzyme activity is substrate concentration. This study aims to isolat and determine the activation and characterization of the substrate as the highest producer of amylase and protease enzymes. Bacillus altitudinis extracted after that found that it could produce amylase and protease enzymes from a qualitative screening test of enzyme activity. This crude extract of the enzyme was measured, its protein content by the Lowry method. The amylase enzyme activity testing by the DNS method at 1%, 1.25%, and 1.5% substrate concentrations and the protease enzyme activity by the Bergmeyer method at 1%, 1.5%, and 2% substrate concentrations. The results showed that the isolats of Bacillus altitudinis bacteria had amylase and protease enzyme activity. The highest enzyme-substrate characterization was at 1.5% starch concentration with amylase enzyme activity of 0.0449 U/mL and 2% casein concentration of 0.8538 U/mL for protease enzyme activity. Keyword : Amylase, Protease, Bacillus altitudinis, Concentrate substrate

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Amylase, Protease, Bacillus altitudinis, Concentrate substrate
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	29 Jul 2022 07:47
Last Modified:	29 Jul 2022 07:47
URI:	http://repo.setiabudi.ac.id/id/eprint/5022

Actions (login required)

View Item