ISOLASI DAN KARAKTERISASI ISOLAT BAKTERI AIR LIMBAH TAMBANG BATUBARA PENGHASIL ENZIM PROTEASE DARI BAKUNGAN TENGGARONG KALIMANTAN TIMUR

Istiqomah, Istiqomah (2018) ISOLASI DAN KARAKTERISASI ISOLAT BAKTERI AIR LIMBAH TAMBANG BATUBARA PENGHASIL ENZIM PROTEASE DARI BAKUNGAN TENGGARONG KALIMANTAN TIMUR. Skripsi thesis, Universitas Setia Budi Surakarta.

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Abstract

Microorganisms from the water is diverse and has an important role as the provider of this species, nutrient elements for living beings in the water and also as a producer of enzymes. The role of the enzyme is essential for food industry, medicine, agriculture, and livestock. One of the enzymes that are widely dispersed and its role quite well in the industry is a protease. Protease is an enzyme which can decipher or break down protein. This enzyme would catalyze the hydrolysis reactions, reactions involving elements of water on bonding of specific substrates. This research aims to isolate and identify bacteria obtained at sewage water the East Kalimantan coal Tenggarong capable of producing the enzyme protease. This research is a descriptive qualitative research. The data obtained are presented in descriptive macroscopic, microscopic identification include, qualitative protease activity assay. Test potential proteolytic qualitatively by measuring the diameter of the clear zone in the media Skim Milk Agar. Results isolation taken five bacterial isolates capable of producing clear zone on a substrate of Skim Milk Agar. Based on macroscopic tests all bacteria have the color, shape, margin, and nearly the same elevation. On coloring grams staining capsule and staining spores all bacteria classified as Gram-negative, encapsulated and there are spores. Proteolytic activity of test results on average at 3 times the sampling of 5 isolates that are able to produce a clear zone surrounding the colony namely (14.53 mm) ALT1, ALT2 (6.63 mm), ALT3 (7.36), ALT4 (8.3 mm), ALT5 (8.6 mm). ALT1 isolates have the highest proteolytic index value. Key words: isolation, identification, bacteria, waste, coal, protease

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	isolation, identification, bacteria, waste, coal, protease
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	Tifany Nur Arfiana
Date Deposited:	23 Feb 2019 02:46
Last Modified:	23 Feb 2019 02:46
URI:	http://repo.setiabudi.ac.id/id/eprint/531

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