UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK ETANOL DAUN KEMANGI (Ocimum sanctum L.) DENGAN DAUN SIRSAK (Annona muricata L.) TERHADAP BAKTERI Staphylococcus aureus

Marliana, Adisa (2024) UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK ETANOL DAUN KEMANGI (Ocimum sanctum L.) DENGAN DAUN SIRSAK (Annona muricata L.) TERHADAP BAKTERI Staphylococcus aureus. Skripsi thesis, Universitas Setia Budi.

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Abstract

MARLIANA. A, 2023, UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK ETANOL DAUN KEMANGI (Ocimum sanctum L.) DENGAN DAUN SIRSAK (Annona muricata L.) TERHADAP BAKTERI Staphylococcus aureus, SKRIPSI, PROGRAM STUDI S1 FARMASI, FAKULTAS FARMASI, UNIVERSITAS SETIA BUDI, SURAKARTA. Dibimbing oleh Dr. Apt. Ismi Rahmawati, M.Si. dan apt. Fitri Kurniasari, M. Farm. Bakteri Staphylococcus aureus adalah bakteri flora normal yang dapat menyebabkan penyakit infeksi pada tubuh. Daun kemangi (Ocimum sanctum L) dan daun sirsak (Annona muricata L) memiliki kandungan senyawa yang dapat digunakan sebagai antibakteri. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri kombinasi ekstrak etanol daun kemangi dengan daun sirsak terhadap bakteri S. aureus. Penelitian ini menggunakan metode maserasi pada daun kemangi dan daun sirsak menggunakan pelarut etanol 96%. Metode dilusi digunakan untuk menetapkan nilai KHM dan KBM berdasarkan perbandingan yang paling efektif dalam membunuh bakteri dengan seri pengenceran mulai dari 50%; 25%; 12,5%; 6,25%; 3,12%; 1,56%; 0,78%; 0,39%; 0,19%; 0,09%. Metode difusi dengan kertas cakram menggunakan perbandingan (1:1); (1:2); (2:1). Aktivitas antibakteri terbaik dilakukan uji pola kombinasi menggunakan metode pita kertas. Analisis data menggunakan SPSS dengan nilai signifikan (p>0,05). Penelitian menunjukkan bahwa ekstrak daun kemangi memiliki nilai KBM 12,5% dan daun sirsak memiliki KBM 12,5%. Kombinasi (1:1) memiliki diameter 20,26±1,40. Kombinasi (1:2) memiliki diameter 26,56±1,35. Kombinasi (2:1) memiliki diameter 19,83±1,00. Kombinasi terbaik pada konsentrasi (1:2). Efek kombinasi antar ekstrak adalah adiktif MARLIANA. A, 2023, ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT COMBINATION OF BASIL LEAVES (Ocimum sanctum L.) WITH SOURSOP LEAVES (Annona muricata L.) AGAINTS THE staphylococcus aureus BACTERIA, UNDERGRADUATETHESIS, BACHELOR OF PHARMACY STUDY PROGRAM, FACULTY OF PHARMACY, SETIA BUDI UNIVERSITY, SURAKARTA. Supervised by Dr. Apt. Ismi Rahmawati, M.Sc. and apt. Fitri Kurniasari, M. Farm. \ Staphylococcus aureus bacteria are part of the normal flora and can spread infectious illnesses throughout the body. Basil leaves (Ocimum sanctum L.) and soursop leaves (Annona muricata L.) both contain substances that can be utilized as antibacterials. The purpose of this study was to examine the antibacterial activity of a combination of basil leaf ethanol extract and soursop leaves against S. aureus bacteria. This study employed a maceration method on basil and soursop leaves with a 96% ethanol solvent. The dilution method was used to calculate the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values based on the most effective ratio in killing bacteria, with a dilution series beginning at 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.39%, 0.19%, and 0.09%. The diffusion method using paper discs employed the following ratios: (1:1), (1:2), and (2:1). The best antibacterial activity was carried out through a combination pattern test using the paper tape method. Data analysis used SPSS with significant values (p>0.05). According to research, basil leaf extract had a MBC value of 12.5% and soursop leaves had a MBC value of 12.5%. The combination (1:1) had a diameter of 20.26 ± 1.40. The combination (1:2) had a diameter of 26.56 ± 1.35. The combination (2:1) had a diameter of 19.83 ± 1.00. The best combination in concentration (1:2). Therefore, the combined effects of the extracts were addictive.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Antibakteri, Staphylococcus aureus, daun kemangi, daun sirsak, ekstraksi. Antibacterial, Staphylococcus aureus, Basil Leaves, Soursop Leaves, Extraction.
Subjects:	Q Science > Q Science (General) R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	USB
Date Deposited:	20 Jun 2024 01:45
Last Modified:	20 Jun 2024 01:45
URI:	http://repo.setiabudi.ac.id/id/eprint/6216

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