UJI SITOTOKSIK EKSTRAK ETANOL DAN FRAKSI n-HEKSANA UMBI BIDARA UPAS (Merremia mammosa (Lour.) Haller f.) TERHADAP SEL KANKER PAYUDARA T47D

Setyara, Merisa (2016) UJI SITOTOKSIK EKSTRAK ETANOL DAN FRAKSI n-HEKSANA UMBI BIDARA UPAS (Merremia mammosa (Lour.) Haller f.) TERHADAP SEL KANKER PAYUDARA T47D. Skripsi thesis, Universitas Setia Budi Surakarta.

	Text INTISARI DAN ABSTRAK.pdf Download (161kB)
	Text SKRIPSI PDF.pdf Download (3MB)

Abstract

Breast cancer in women is the first rank compared to other cancer cases. Bidara upas tuber (Merremia mammosa (Lour.) Haller f.) was empirically used to treat tumors. This study aimed to determine the cytotoxic activity of ethanol extract and n-hexane fraction bidara upas tuber against breast cancer T47D cells and determine the selectivity index of ethanol extract against vero cells. Bidara upas tuber was macerated by 96% ethanol and was fractionated by n-hexane. The cytotoxic activity test of ethanol extract and n-hexane fraction of bidara upas tuber was done using Microculture Tetrazolium Technique (MTT) method with a series for extract concentration 1000; 500; 250; 125; 62.5; 31.2; 15.6 µg/ml and for fraction of n-hexane of 500; 250; 125; 62.5; 31.2; 15.6; 7.8 µg/ml. Cell death was calculated determine used linear regression equation between log concentration cell with % viability. The selectivity of cytotoxic activity was known by selectivity index equation, which was IC50 of vero cells proportionate with IC50 of T47D cells. The results of cytotoxic activity of ethanol extract of bidara upas tuber have IC50 value of 165,163 µg/ml (> 100 µg/ml), it was not showed potent activities and IC50 value was showed fraction of n-hexane bidara upas of 28.567 µg/ml(< 100 µg/ml), it was showed potent active against breast cancer cells T47D. Selectivity index of ethanol extract in against vero cells was gotten value of 4.473. Key words : bidara upas tuber, T47D cells, cytotoxic, selectivity index

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	bidara upas tuber, T47D cells, cytotoxic, selectivity index
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Prodi S1 Farmasi
Depositing User:	magdalena kartika ningsih
Date Deposited:	23 Feb 2019 03:31
Last Modified:	23 Feb 2019 03:31
URI:	http://repo.setiabudi.ac.id/id/eprint/748

Actions (login required)

View Item