PERBEDAAN PERLAKUAN WAKTU PEWARNAAN HEMATOXYLIN – EOSIN TERHADAP GAMBARAN HISTOLOGI INTI SEL DAN SITOPLASMA HATI AYAM

Rumpuin, Desyana (2023) PERBEDAAN PERLAKUAN WAKTU PEWARNAAN HEMATOXYLIN – EOSIN TERHADAP GAMBARAN HISTOLOGI INTI SEL DAN SITOPLASMA HATI AYAM. Skripsi thesis, Universitas Setia Budi.

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Abstract

RUMPUIN DESYANA, 2023. DIFFERENCES IN TIME TREATMENT OF HEMATOXYLIN – EOSIN STAINING ON HISTOLOGICAL IMAGES OF CELL CELLS AND CYTOPLASM OF CHICKEN LIVER. D4 HEALTH ANALYST STUDY PROGRAM, FACULTY OF HEALTH SCIENCES, SETIA BUDI UNIVERSITY. Histology is the study of the morphology and properties of normal tissues or cells. Histotechnics is a method for making histological preparations from certain specimens through a series of processes until they become preparations ready to be observed or analyzed using a microscope. This study used descriptive analysis of microscopic histological images of the nuclei and cytoplasm of chicken livers that had been stained using Hematoxylin Eosin staining. The purpose of this study was to determine the differences in the results of staining of histological preparations of cell nuclei and cytoplasm of chicken liver cells with variations in the duration of Hematoxylin – Eosin staining. The staining stage (staining) was given various treatments with staining duration as follows: Treatment I (control): Hematoxylin staining for 15 minutes, Eosin for 5 minutes. Treatment II: Hematoxylin staining for 20 seconds, Eosin for 20 seconds. Treatment III: Hematoxylin staining for 20 minutes, Eosin for 10 minutes. The conclusion of this study is that based on the results of the research and discussion that has been described, it can be concluded that the staining time affects the microscopic appearance of tissue. Staining with Hematoxylin 15 minutes and Eosin 5 minutes gave a good microscopic picture, while for Hematoxylin 20 seconds Eosin 20 seconds and Hematoxylin 20 minutes Eosin 10 minutes stained gave a poor microscopic picture because the staining time was fast and long. So the length of staining time is very influential on the results of tissue microscopy.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: histologi hepar ayam, inti sel, sitoplasma, Hematoxylin Eosin, waktu pewarnaan, chicken liver histology, cell nucleus, cytoplasm, Hematoxylin Eosin, coloring time
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Ilmu Kesehatan > Prodi D4 Analis Kesehatan
Depositing User: USB
Date Deposited: 08 Oct 2024 04:39
Last Modified: 08 Oct 2024 04:39
URI: http://repo.setiabudi.ac.id/id/eprint/6394

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